@article{0b34ac59f3be4ec1970e4d1c8a4fbb1d,
title = "The dark proteome: translation from noncanonical open reading frames",
abstract = "Omics-based technologies have revolutionized our understanding of the coding potential of the genome. In particular, these studies revealed widespread unannotated open reading frames (ORFs) throughout genomes and that these regions have the potential to encode novel functional (micro-)proteins and/or hold regulatory roles. However, despite their genomic prevalence, relatively few of these noncanonical ORFs have been functionally characterized, likely in part due to their under-recognition by the broader scientific community. The few that have been investigated in detail have demonstrated their essentiality in critical and divergent biological processes. As such, here we aim to discuss recent advances in understanding the diversity of noncanonical ORFs and their roles, as well as detail biologically important examples within the context of the mammalian genome.",
keywords = "CRISPR, microproteins, noncanonical ORFs, ribosome profiling, short ORFs, translation",
author = "Wright, {Bradley W.} and Zixin Yi and Weissman, {Jonathan S.} and Jin Chen",
note = "Funding Information: We thank Se{\'a}n E. O'Leary (University of California, Riverside) and Jingchuan Luo (Whitehead Institute, MIT) for critical reading of our review. This work was supported by the University of Texas Southwestern Medical Center Endowed Scholars Program, a National Institute of Health grant R00GM134154, and a Cancer Prevention and Research Institute of Texas grant RR200095 (to J.C.). J.S.W. is a Howard Hughes Medical Institute (HHMI) Investigator. We apologize to the authors of studies that we could not cite here due to limitations of space. J.S.W. declares outside interest in 5 AM Venture, Amgen, Chroma Medicine, KSQ Therapeutics, Maze Therapeutics, Tenaya Therapeutics, Tessera Therapeutics, Third Rock Ventures, and Velia Therapeutics. J.C. consults for Velia Therapeutics. Funding Information: We thank Se{\'a}n E. O{\textquoteright}Leary (University of California, Riverside) and Jingchuan Luo (Whitehead Institute, MIT) for critical reading of our review. This work was supported by the University of Texas Southwestern Medical Center Endowed Scholars Program, a National Institute of Health grant R00GM134154, and a Cancer Prevention and Research Institute of Texas grant RR200095 (to J.C.). J.S.W. is a Howard Hughes Medical Institute (HHMI) Investigator . We apologize to the authors of studies that we could not cite here due to limitations of space. Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",
year = "2022",
month = mar,
doi = "10.1016/j.tcb.2021.10.010",
language = "English (US)",
volume = "32",
pages = "243--258",
journal = "Trends in Cell Biology",
issn = "0962-8924",
publisher = "Elsevier Limited",
number = "3",
}