@article{ab64c93a940349d49186ca8cd5ceb320,
title = "The conserved misshapen-warts-yorkie pathway acts in enteroblasts to regulate intestinal stem cells in drosophila",
abstract = "Similar to the mammalian intestine, the Drosophila adult midgut has resident stem cells that support growth and regeneration. How the niche regulates intestinal stem cell activity in both mammals and flies is not well understood. Here, we show that the conserved germinal center protein kinase Misshapen restricts intestinal stem cell division by repressing the expression of the JAK-STAT pathway ligand Upd3 in differentiating enteroblasts. Misshapen, a distant relative to the prototypic Warts activating kinase Hippo, interacts with and activates Warts to negatively regulate the activity of Yorkie and the expression of Upd3. The mammalian Misshapen homolog MAP4K4 similarly interacts with LATS (Warts homolog) and promotes inhibition of YAP (Yorkie homolog). Together, this work reveals thatthe Misshapen-Warts-Yorkie pathway acts in enteroblasts to control niche signaling to intestinal stem cells. These findings also provide a model in which to study requirements for MAP4K4-related kinases in MST1/2-independent regulation of LATS and YAP.",
author = "Qi Li and Shuangxi Li and Sebastian Mana-Capelli and RothFlach, {Rachel J.} and Danai, {Laura V.} and Alla Amcheslavsky and Yingchao Nie and Satoshi Kaneko and Xiaohao Yao and Xiaochu Chen and Cotton, {Jennifer L.} and Junhao Mao and Dannel McCollum and Jin Jiang and Czech, {Michael P.} and Lan Xu and Ip, {Y. Tony}",
note = "Funding Information: We acknowledge the Vienna Drosophila RNAi Center and the Bloomington Drosophila Stock Center for the transgenic RNAi lines. We thank Drs. Herv{\'e} Agaisse, DJ Pan, Huaqi Jiang, Steven Hou, Jianghang Jia, Lei Zhang, and Xiaohang Yang for fly stocks and reagents. Y.T.I. is supported by an NIH grant (DK83450) and is a member of the UMass DERC (DK32520), the UMass Center for Clinical and Translational Science (UL1TR000161), and the Guangdong Innovative Research Team Program (201001Y0104789252). J.M. is supported by an American Cancer Society grant (120376-RSG-11-040-01-DDC). J.J. is supported by NIH grants (GM61269 and GM67045), a Cancer Prevention & Research Institute of Texas (CPRIT) grant (RP130542), a Welch Foundation grant (I-1603), and a National Natural Science Foundation of China (NSFC) grant (31328017). These studies were also supported by grants to M.P.C. from the NIH (DK030898) and the International Research Alliance of the Novo Nordisk Foundation Center for Metabolic Research. D.M. was supported by NIH grant GM058406-14. Publisher Copyright: {\textcopyright} 2014 Elsevier Inc.",
year = "2014",
month = nov,
day = "10",
doi = "10.1016/j.devcel.2014.09.012",
language = "English (US)",
volume = "31",
pages = "291--304",
journal = "Developmental cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "3",
}