Abstract
Four AP2/EREBP genes encoding putative ethylene-responsive element binding factor (ERF)/AP2 domains were cloned from Brassica napus, and these genes could be induced by low temperature, ethylene, drought, high salinity, abscisic acid and jasmonate treatments. These four genes, named BnDREBIII-1 to BnDREBIII-4, were highly homologous and the 37th amino acid was the only difference among their ERF/AP2 domains. BnDREBIII-1 was demonstrated to be able to bind to both dehydration-responsive element and the GCC box and transactivate the expression of downstream genes, while BnDREBIII-4 could bind neither. Further results suggested that Ala37 might play a crucial role in the DNA binding or the stability of the ERF/AP2 domain.
Original language | English (US) |
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Pages (from-to) | 1303-1308 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 580 |
Issue number | 5 |
DOIs | |
State | Published - Feb 20 2006 |
Keywords
- BnDREBIII
- Brassica napus
- Dehydration-responsive element
- ERF/AP2 domain
- GCC box
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology