The collagen recognition sequence for fibroblasts depends on collagen topography

Frederick Grinnell; Shigenori Nakagawa; Chin Han Ho

doi:10.1016/0014-4827(89)90270-X

The collagen recognition sequence for fibroblasts depends on collagen topography

Frederick Grinnell, Shigenori Nakagawa, Chin Han Ho

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

We found that the peptide GlyArgGlyGluSerPro (GRGESP) inhibited spreading of human fibroblasts inside collagen gels and markedly decreased gel contraction, but this peptide had no effect on cell spreading on collagen-coated surfaces. On the other hand, the peptide GlyArgGlyAspSerPro (GRGDSP), which inhibited cell spreading on collagen-coated surfaces, did not inhibit cell spreading within collagen gels and was a less effective inhibitor of collagen gel contraction than GRGESP. Based on these findings, we conclude that human fibroblasts can interact with different collagen cell recognition sequences depending upon topographical organization of the collagen.

Original language	English (US)
Pages (from-to)	668-672
Number of pages	5
Journal	Experimental Cell Research
Volume	182
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(89)90270-X
State	Published - Jun 1989

ASJC Scopus subject areas

Cell Biology

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10.1016/0014-4827(89)90270-X

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@article{89e9b24d88da4da7b4925f424fb97e75,

title = "The collagen recognition sequence for fibroblasts depends on collagen topography",

abstract = "We found that the peptide GlyArgGlyGluSerPro (GRGESP) inhibited spreading of human fibroblasts inside collagen gels and markedly decreased gel contraction, but this peptide had no effect on cell spreading on collagen-coated surfaces. On the other hand, the peptide GlyArgGlyAspSerPro (GRGDSP), which inhibited cell spreading on collagen-coated surfaces, did not inhibit cell spreading within collagen gels and was a less effective inhibitor of collagen gel contraction than GRGESP. Based on these findings, we conclude that human fibroblasts can interact with different collagen cell recognition sequences depending upon topographical organization of the collagen.",

author = "Frederick Grinnell and Shigenori Nakagawa and Ho, {Chin Han}",

note = "Funding Information: We thank Drs. William Snell, George Bloom, and Richard Anderson for their helpful suggestions. This researchw as supportedb y grants from the National Institute of General Medical Sciences and National Cancer Institute.",

year = "1989",

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doi = "10.1016/0014-4827(89)90270-X",

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T1 - The collagen recognition sequence for fibroblasts depends on collagen topography

AU - Grinnell, Frederick

AU - Nakagawa, Shigenori

AU - Ho, Chin Han

N1 - Funding Information: We thank Drs. William Snell, George Bloom, and Richard Anderson for their helpful suggestions. This researchw as supportedb y grants from the National Institute of General Medical Sciences and National Cancer Institute.

PY - 1989/6

Y1 - 1989/6

N2 - We found that the peptide GlyArgGlyGluSerPro (GRGESP) inhibited spreading of human fibroblasts inside collagen gels and markedly decreased gel contraction, but this peptide had no effect on cell spreading on collagen-coated surfaces. On the other hand, the peptide GlyArgGlyAspSerPro (GRGDSP), which inhibited cell spreading on collagen-coated surfaces, did not inhibit cell spreading within collagen gels and was a less effective inhibitor of collagen gel contraction than GRGESP. Based on these findings, we conclude that human fibroblasts can interact with different collagen cell recognition sequences depending upon topographical organization of the collagen.

AB - We found that the peptide GlyArgGlyGluSerPro (GRGESP) inhibited spreading of human fibroblasts inside collagen gels and markedly decreased gel contraction, but this peptide had no effect on cell spreading on collagen-coated surfaces. On the other hand, the peptide GlyArgGlyAspSerPro (GRGDSP), which inhibited cell spreading on collagen-coated surfaces, did not inhibit cell spreading within collagen gels and was a less effective inhibitor of collagen gel contraction than GRGESP. Based on these findings, we conclude that human fibroblasts can interact with different collagen cell recognition sequences depending upon topographical organization of the collagen.

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The collagen recognition sequence for fibroblasts depends on collagen topography

Abstract

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