Abstract
In the Neurospora circadian negative feedback loop, WHITE COLLAR 1 (WC-1) and WC-2 form the WC complex that activates frequency (frq) transcription. Here we show that Not1 is a WC-interacting protein and is important for maintaining WC levels. The not1 transcript displays a circadian oscillation with a similar phase as frq. Down-regulation of not1 leads to low levels of WC-1 and WC-2 and a delayed circadian phase as a result of increased protein degradation and increased WC activity. Protein purification of Not1 shows that it is part of the Neurospora Ccr4-Not complex. ccr4 is a clock-controlled gene and is regulated directly by the WC complex. Down-regulation of ccr4 results in a phase delay and period lengthening of the clock. Together, our findings suggest that the Ccr4-Not complex participates in the Neurospora clock function by interacting with and regulating the WC complex.
Original language | English (US) |
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Pages (from-to) | 31002-31009 |
Number of pages | 8 |
Journal | Journal of Biological Chemistry |
Volume | 288 |
Issue number | 43 |
DOIs | |
State | Published - Oct 25 2013 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology