TY - JOUR
T1 - The application of confocal microscopy to the study of living systems
AU - Cavanagh, Harrison D
AU - Petroll, Walter M
AU - Jester, James V.
N1 - Funding Information:
Supported in part by Grant #EY05957 from the National Institutes of Health and by grants from the Teubert Charitable Foundation and the Ford Motor Company Fund. This Paper will be presented in part at the SPIE conference "Visual communications and image processing '93." A shorter version of it will appear (unrefereed) in the conference proceedings.
PY - 1993
Y1 - 1993
N2 - A unique tandem confocal microscope (TSCM) has been developed that permits noninvasive imaging in vivo of the eye and many other organ systems in real time in situ. The application to the study of microphysiological processes in vivo is described and illustrated for the cornea, kidney, liver, epididymis, muscle, and adipose tissue. Novel applications are shown for studying the healing of wounds in four dimensions (x, y, z, t) in single animals over time at the cellular level. Application to clinical diagnostic use in humans is also demonstrated. When combined with Laser Scanning Confocal fluorescence microscopy, the TSCM offers a unique new imaging paradigm for experimental biology and medicine with great potential for use in neuroscience and many other disciplines.
AB - A unique tandem confocal microscope (TSCM) has been developed that permits noninvasive imaging in vivo of the eye and many other organ systems in real time in situ. The application to the study of microphysiological processes in vivo is described and illustrated for the cornea, kidney, liver, epididymis, muscle, and adipose tissue. Novel applications are shown for studying the healing of wounds in four dimensions (x, y, z, t) in single animals over time at the cellular level. Application to clinical diagnostic use in humans is also demonstrated. When combined with Laser Scanning Confocal fluorescence microscopy, the TSCM offers a unique new imaging paradigm for experimental biology and medicine with great potential for use in neuroscience and many other disciplines.
KW - Confocal microscopy
KW - Eye imaging
KW - Ivo
KW - Non-invasive imaging
KW - Ophthalmology imaging
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U2 - 10.1016/S0149-7634(05)80127-7
DO - 10.1016/S0149-7634(05)80127-7
M3 - Article
AN - SCOPUS:58149208370
SN - 0149-7634
VL - 17
SP - 477
EP - 482
JO - Neuroscience and Biobehavioral Reviews
JF - Neuroscience and Biobehavioral Reviews
IS - 4
ER -