Abstract
We measured transforming growth factor (TGF)-β-dependent biomarkers in plasma and in peripheral blood mononuclear cells (PBMCs) to identify suitable pharmacodynamic markers for future clinical trials with TGF-β inhibitors. Forty-nine patients with bone metastasis were enrolled in the study, including patients with breast (n = 23) and prostate cancer (n = 15). Plasma TGF-β1 levels were elevated in more than half of the cancer patients (geometric mean 2.63 ng ml-1) and positively correlated with increased platelet factor 4 (PF4) levels, parathyroid-related protein (PTHrP), von Willebrand Factor (vWF) and interleukin (IL)-10. PBMC were stimulated ex vivo to determine the individual biological variability of an ex vivo assay measuring pSMAD expression. This assay performed sufficiently well to allow its future use in a clinical trial of a TGF-β inhibitor.
Original language | English (US) |
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Pages (from-to) | 217-236 |
Number of pages | 20 |
Journal | Biomarkers |
Volume | 13 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2008 |
Keywords
- Cancer
- Plasma biomarkers
- TGF-β
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Health, Toxicology and Mutagenesis