Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation

Kenji Murakami; Brian J. Gibbons; Ralph E. Davis; Shigeki Nagai; Xin Liu; Philip J J Robinson; Tinghe Wu; Craig D. Kaplan; Roger D. Kornberg

doi:10.1073/pnas.1201448109

Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation

Kenji Murakami, Brian J. Gibbons, Ralph E. Davis, Shigeki Nagai, Xin Liu, Philip J J Robinson, Tinghe Wu, Craig D. Kaplan, Roger D. Kornberg

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

General transcription factor TFIIH, previously described as a 10-subunit complex, is essential for transcription and DNA repair. An eleventh subunit now identified, termed Tfb6, exhibits 45% sequence similarity to human nuclear mRNA export factor 5. Tfb6 dissociates from TFIIH as a heterodimer with the Ssl2 subunit, a DNA helicase that drives promoter melting for the initiation of transcription. Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes.

Original language	English (US)
Pages (from-to)	4816-4821
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	109
Issue number	13
DOIs	https://doi.org/10.1073/pnas.1201448109
State	Published - Mar 27 2012

Keywords

DNA repair
General transcription factor
RNA polymerase II
TFIIK
Transcription pre-initiation complex
Yeast

ASJC Scopus subject areas

General

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10.1073/pnas.1201448109

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Murakami, K., Gibbons, B. J., Davis, R. E., Nagai, S., Liu, X., Robinson, P. J. J., Wu, T., Kaplan, C. D., & Kornberg, R. D. (2012). Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation. Proceedings of the National Academy of Sciences of the United States of America, 109(13), 4816-4821. https://doi.org/10.1073/pnas.1201448109

Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation. / Murakami, Kenji; Gibbons, Brian J.; Davis, Ralph E. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 13, 27.03.2012, p. 4816-4821.

Research output: Contribution to journal › Article › peer-review

Murakami, K, Gibbons, BJ, Davis, RE, Nagai, S, Liu, X, Robinson, PJJ, Wu, T, Kaplan, CD & Kornberg, RD 2012, 'Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 13, pp. 4816-4821. https://doi.org/10.1073/pnas.1201448109

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abstract = "General transcription factor TFIIH, previously described as a 10-subunit complex, is essential for transcription and DNA repair. An eleventh subunit now identified, termed Tfb6, exhibits 45% sequence similarity to human nuclear mRNA export factor 5. Tfb6 dissociates from TFIIH as a heterodimer with the Ssl2 subunit, a DNA helicase that drives promoter melting for the initiation of transcription. Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes.",

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T1 - Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation

AU - Murakami, Kenji

AU - Gibbons, Brian J.

AU - Davis, Ralph E.

AU - Nagai, Shigeki

AU - Liu, Xin

AU - Robinson, Philip J J

AU - Wu, Tinghe

AU - Kaplan, Craig D.

AU - Kornberg, Roger D.

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KW - DNA repair

KW - General transcription factor

KW - RNA polymerase II

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KW - Transcription pre-initiation complex

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Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation

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