TY - JOUR
T1 - Tetracycline-controllable selection of CD4+ T cells
T2 - Half-life and survival signals in the absence of major histocompatibility complex class II molecules
AU - Witherden, Deborah
AU - Van Oers, Nicolai
AU - Waltzinger, Caroline
AU - Weiss, Arthur
AU - Benoist, Christophe
AU - Mathis, Diane
PY - 2000/1/17
Y1 - 2000/1/17
N2 - A system that allows the study, in a gentle fashion, of the role of MHC molecules in naive T cell survival is described. Major histocompatibility complex class II-deficient mice were engineered to express Eα chains only in thymic epithelial cells in a tetracycline (tet)-controllable manner. This resulted in tet-responsive display of cell surface E complexes, positive selection of CD4+8- thymocytes, and generation of a CD4+ T cell compartment in a class II-barren periphery. Using this system, we have addressed two unresolved issues: the half-life of naive CD4+ T cells in the absence of class II molecules (3-4 wk) and the early signaling events associated with class II molecule engagement by naive CD4+ T cells (partial CD3 ζ chain phosphorylation and ZAP-70 association).
AB - A system that allows the study, in a gentle fashion, of the role of MHC molecules in naive T cell survival is described. Major histocompatibility complex class II-deficient mice were engineered to express Eα chains only in thymic epithelial cells in a tetracycline (tet)-controllable manner. This resulted in tet-responsive display of cell surface E complexes, positive selection of CD4+8- thymocytes, and generation of a CD4+ T cell compartment in a class II-barren periphery. Using this system, we have addressed two unresolved issues: the half-life of naive CD4+ T cells in the absence of class II molecules (3-4 wk) and the early signaling events associated with class II molecule engagement by naive CD4+ T cells (partial CD3 ζ chain phosphorylation and ZAP-70 association).
KW - Inducible expression
KW - Lymph node
KW - T lymphocyte
KW - Transgenic
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U2 - 10.1084/jem.191.2.355
DO - 10.1084/jem.191.2.355
M3 - Article
C2 - 10637279
AN - SCOPUS:0034677036
SN - 0022-1007
VL - 191
SP - 355
EP - 364
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 2
ER -