@article{62d835e1902842dc8adeb1f28ceb4de8,
title = "Telomerase activity in ordinary meningiomas predicts poor outcome",
abstract = "Telomerase, the enzyme that stabilizes telomere length, is reactivated with almost all cancer types, and it may be necessary for unlimited cell proliferation. Assessment of malignancy in ordinary meningiomas is inconclusive because no clear-cut correlation exists between aggressive clinical behavior and histological features or karyotypic abnormalities. We analyzed telomerase activity in 52 cases of meningioma by using the highly sensitive telomeric repeat amplification protocol and then compared clinical behavior in telomerase-positive and -negative ordinary meningiomas. Twenty- six of the 52 tumors (50%) had detectable telomerase activity. Twenty-one of the 22 neoplasms classified as malignant or atypical (95%) had detectable telomerase activity, and these tumors generally had a poor outcome. Interestingly, 5 of 30 ordinary (morphologically benign) meningiomas (17%) also showed detectable telomerase activity. Of the 5 patients with telomerase-positive ordinary meningiomas, 3 had rapid regrowth of the tumor despite gross total resection. The remaining 2 patients also had other primary malignancies. We observed a highly significant correlation in ordinary meningiomas between the presence of telomerase activity and a poor prognosis for the patient (P = .0002). Telomerase activity in benign meningiomas is clinically relevant because the presence of the enzyme suggests that these benign-appearing tumors may contain a population of immortal cells. The detection of telomerase activity may help to identify benign meningiomas that would be more likely to continue to grow and to recur clinically if surgical resection were incomplete.",
keywords = "immortality, meningioma, proliferation, telomerase",
author = "Langford, {Lauren A.} and Piatyszek, {Mieczyslaw A.} and Ruishu Xu and Schold, {S. Clifford} and Wright, {Woodring E.} and Shay, {Jerry W.}",
note = "Funding Information: From the Department of Pathology (Neuropathology), the University of Texas M.D. Anderson Cancer Center, Houston, TX; the Departments of Cell Biology and Neurosciences, and Neurology, the University of Texas Southwestern Medical Center, Dallas, TX; and Geron Corporation, Menlo Park, CA. Accepted for publication August 9, 1996. Supported by research grants from the University of Texas M.D. Anderson Cancer Center Patient Referral Service (Houston, TX) (L.A.L.) and Geron Corporation (Menlo Park, CA) (M.A.P.,J.W.S.). Address correspondence and reprint requests to Lauren A. Lang-ford, MD, Department of Pathology, Box 85, M.D. Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030. Copyright {\textcopyright} 1997 by W.B. Saunders Company 0046-8177/97/2804-001155.00/0 correlation in ordinary meningiomas between the presence of telomerase activity and a poor prognosis for the patient (P = .0002). Telomerase activity in benign meningiomas is clinically relevant because the presence of the enzyme suggests that these benign-appearing tumors may contain a population of immortal cells. The detection of telomerase activity may help to identify benign meningiomas that would be more likely to continue to grow and to recur clinically if surgical resection were incomplete. HUM PATHOL 28:416 --420. Copyright {\textcopyright} 1997 by W.B. Saunders Company Key words: meningioma, telomerase, immortality, proliferation. Abbreviations: BCA, bicinchoninic acid; CHAPS, 3-\[(3-cholamidopropyl)-dlmethyl-ammonio\]-l-propanesulfonate;D EPC, diethyl pyrocarbonate; DPBS, Dnibecco's phosphate buffered saline; EGTA, ethylene glycol-bis(/~-aminoethyl ether)-N,N,N',N'-tetra-acetic acid; PCR, polymerase chain reaction; RNase, ribonuclease; TRAP, telomeric repeat amplification protocol; TRIS, tris(hydroxymethyl) aminomethane; HC1, hydrochloric acid.",
year = "1997",
doi = "10.1016/S0046-8177(97)90029-0",
language = "English (US)",
volume = "28",
pages = "416--420",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "4",
}