TY - JOUR
T1 - Synthetic molecules as antibody replacements
AU - Kodadek, Thomas
AU - Reddy, M. Muralidhar
AU - Olivos, Hernando J.
AU - Bachhawat-Sikder, Kiran
AU - Alluri, Prasanna G.
PY - 2004/9
Y1 - 2004/9
N2 - Antibodies are by far the most versatile, valuable, and widely used protein-binding agents. They are essential tools in biological research and are increasingly being developed as therapeutic reagents. However, antibodies have a number of practical limitations, and it would be desirable in many applications to replace them with simpler, more robust synthetic molecules. Unfortunately, synthetic protein-binding agents rarely exhibit the high affinity and specificity typical of a good antibody. This article reviews efforts to overcome these limitations and to develop a facile, high-throughput methodology for the isolation of synthetic protein ligands with antibody-like binding characteristics.
AB - Antibodies are by far the most versatile, valuable, and widely used protein-binding agents. They are essential tools in biological research and are increasingly being developed as therapeutic reagents. However, antibodies have a number of practical limitations, and it would be desirable in many applications to replace them with simpler, more robust synthetic molecules. Unfortunately, synthetic protein-binding agents rarely exhibit the high affinity and specificity typical of a good antibody. This article reviews efforts to overcome these limitations and to develop a facile, high-throughput methodology for the isolation of synthetic protein ligands with antibody-like binding characteristics.
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U2 - 10.1021/ar030145l
DO - 10.1021/ar030145l
M3 - Article
C2 - 15379586
AN - SCOPUS:4644297575
SN - 0001-4842
VL - 37
SP - 711
EP - 718
JO - Accounts of Chemical Research
JF - Accounts of Chemical Research
IS - 9
ER -