Synthesis of dye conjugates to visualize the cancer cells using fluorescence microscopy

Yang Pu, Rui Tang, Jianpeng Xue, W. B. Wang, Baogang Xu, S. Achilefu

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


The clinical diagnosis of most cancers is based on evaluation of histology microscopic slides to view the size and shape of cellular nuclei and morphological structure of tissue. To achieve this goal for in vivo and in-deep tissues, near infrared dyes-bovine serum albumin and immunoglobulin G conjugates were synthesized. The spectral study shows that the absorption and fluorescence of the dye conjugates are in the "tissue optical window" spectral ranges between 650 and 900 nm. The internalization and pinocytosis of the synthesized compounds were investigated at cell level using fluorescence microscopy to obtain the optimal concentration and staining time.

Original languageEnglish (US)
Pages (from-to)2345-2351
Number of pages7
JournalApplied Optics
Issue number11
StatePublished - Apr 10 2014
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering


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