Synaptotagmin regulation of coated pit assembly

Christine Von Poser, Jimmy Z. Zhang, Chieko Mineo, Wei Ding, Yunshu Ying, Thomas C. Südhof, Richard G W Anderson

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


Synaptotagmins bind clathrin AP-2 with high affinity via their second C2 domain, which indicates they are involved in coated pit function. We now report that expression of synaptotagmins lacking either the second C2 domain or the entire cytoplasmic region potently inhibit endocytosis. Inhibition was dependent on two intramembrane cysteine residues that were found to be essential for synaptotagmin oligomerization. Cells expressing the wild-type, but not the mutant, truncated synaptotagmin fragment had a reduced number of clathrin-coated pits. These results suggest that the formation of synaptotagmin multimers is an important step in the regulation of coated pit assembly.

Original languageEnglish (US)
Pages (from-to)30916-30924
Number of pages9
JournalJournal of Biological Chemistry
Issue number40
StatePublished - Oct 6 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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