Synaptotagmin 7 splice variants differentially regulate synaptic vesicle recycling

Tuhin Virmani; Weiping Han; Xinran Liu; Thomas C. Südhof; Ege T. Kavalali

doi:10.1093/emboj/cdg514

Synaptotagmin 7 splice variants differentially regulate synaptic vesicle recycling

Tuhin Virmani, Weiping Han, Xinran Liu, Thomas C. Südhof, Ege T. Kavalali

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

The speed of synaptic vesicle recycling determines the efficacy of neurotransmission during repetitive stimulation. Synaptotagmins are synaptic C₂-domain proteins that are involved in exocytosis, but have also been linked to endocytosis. We now demonstrate that upon expression in transfected neurons, a short splice variant of synaptotagmin 7 that lacks C ₂-domains accelerates endocytic recycling of synaptic vesicles, whereas a longer splice variant that contains C₂-domains decelerates recycling. These results suggest that alternative splicing of synaptotagmin 7 acts as a molecular switch, which targets vesicles to fast and slow recycling pathways.

Original language	English (US)
Pages (from-to)	5347-5357
Number of pages	11
Journal	EMBO Journal
Volume	22
Issue number	20
DOIs	https://doi.org/10.1093/emboj/cdg514
State	Published - Oct 15 2003

Keywords

FM1-43
Hippocampal synapse
Kiss and run
Synaptic vesicle recycling
Synaptotagmin 7

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1093/emboj/cdg514

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title = "Synaptotagmin 7 splice variants differentially regulate synaptic vesicle recycling",

abstract = "The speed of synaptic vesicle recycling determines the efficacy of neurotransmission during repetitive stimulation. Synaptotagmins are synaptic C2-domain proteins that are involved in exocytosis, but have also been linked to endocytosis. We now demonstrate that upon expression in transfected neurons, a short splice variant of synaptotagmin 7 that lacks C 2-domains accelerates endocytic recycling of synaptic vesicles, whereas a longer splice variant that contains C2-domains decelerates recycling. These results suggest that alternative splicing of synaptotagmin 7 acts as a molecular switch, which targets vesicles to fast and slow recycling pathways.",

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T1 - Synaptotagmin 7 splice variants differentially regulate synaptic vesicle recycling

AU - Virmani, Tuhin

AU - Han, Weiping

AU - Liu, Xinran

AU - Südhof, Thomas C.

AU - Kavalali, Ege T.

PY - 2003/10/15

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AB - The speed of synaptic vesicle recycling determines the efficacy of neurotransmission during repetitive stimulation. Synaptotagmins are synaptic C2-domain proteins that are involved in exocytosis, but have also been linked to endocytosis. We now demonstrate that upon expression in transfected neurons, a short splice variant of synaptotagmin 7 that lacks C 2-domains accelerates endocytic recycling of synaptic vesicles, whereas a longer splice variant that contains C2-domains decelerates recycling. These results suggest that alternative splicing of synaptotagmin 7 acts as a molecular switch, which targets vesicles to fast and slow recycling pathways.

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KW - Kiss and run

KW - Synaptic vesicle recycling

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Synaptotagmin 7 splice variants differentially regulate synaptic vesicle recycling

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