Surface Ig isotypes on cells responding to lipopolysaccharide by IgM and IgG secretion

E. S. Gronowicz, C. Doss, F. Assisi, E. S. Vitetta, R. L. Coffman, S. Strober

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Using the fluorescence-activated cell sorter (FACS), we have investigated that the Ig isotypes present on murine B cells, which can be polyclonally activated by lipopolysaccharide (LPS) in low cell density cultures. The LPS response was partly inhibited as a result of staining with anti-IgD and anti-IgM reagents, but not with anti-IgG reagents. The IgM +, IgD +, or IgG - fractionated cell populations gave both an IgM and an IgG response comparable to controls, whereas the response of the IgM -, IgD - cells was 5- to 20-fold lower. IgG - cells separated 1 day after LPS stimulation could still mount an IgM and IgG response indistinguishable from controls at the peak of the response. It is concluded that IgM +, IgD +, IgG - cells constitute the major LPS-sensitive cell population in the low cell density culture system and that IgG is not a necessary cell surface isotype for precursors of IgG-secreting cells.

Original languageEnglish (US)
Pages (from-to)2049-2056
Number of pages8
JournalJournal of Immunology
Issue number5
StatePublished - 1979

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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