TY - JOUR
T1 - Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells
AU - Kaznacheyeva, Elena
AU - Glushankova, Lyuba
AU - Bugaj, Vladislav
AU - Zimina, Olga
AU - Skopin, Anton
AU - Alexeenko, Vadim
AU - Tsiokas, Leonidas
AU - Bezprozvanny, Ilya
AU - Mozhayeva, Galina N.
PY - 2007/8/10
Y1 - 2007/8/10
N2 - In most non-excitable cells, calcium (Ca2+) release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca 2+ stores is coupled to Ca2+ influx through the plasma membrane Ca2+ channels whose molecular composition is poorly understood. Several members of mammalian TRP-related protein family have been implicated to both receptor- and store-operated Ca2+ influx. Here we investigated the role of the native transient receptor potential 3 (TRPC3) homologue in mediating the store- and receptor-operated calcium entry in A431 cells. We show that suppression of TRPC3 protein levels by small interfering RNA (siRNA) leads to a significant reduction in store-operated calcium influx without affecting the receptor-operated calcium influx. With single-channel analysis, we further demonstrate that reduction of TRPC3 levels results in suppression of specific subtype of store-operated calcium channels and activation of store-independent channels. Our data suggest that TRPC3 is required for the formation of functional store-operated channels in A431 cells.
AB - In most non-excitable cells, calcium (Ca2+) release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca 2+ stores is coupled to Ca2+ influx through the plasma membrane Ca2+ channels whose molecular composition is poorly understood. Several members of mammalian TRP-related protein family have been implicated to both receptor- and store-operated Ca2+ influx. Here we investigated the role of the native transient receptor potential 3 (TRPC3) homologue in mediating the store- and receptor-operated calcium entry in A431 cells. We show that suppression of TRPC3 protein levels by small interfering RNA (siRNA) leads to a significant reduction in store-operated calcium influx without affecting the receptor-operated calcium influx. With single-channel analysis, we further demonstrate that reduction of TRPC3 levels results in suppression of specific subtype of store-operated calcium channels and activation of store-independent channels. Our data suggest that TRPC3 is required for the formation of functional store-operated channels in A431 cells.
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U2 - 10.1074/jbc.M608378200
DO - 10.1074/jbc.M608378200
M3 - Article
C2 - 17569672
AN - SCOPUS:34548173034
SN - 0021-9258
VL - 282
SP - 23655
EP - 23662
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 32
ER -