Suppression of human bladder cancer growth by increased expression of C- CAM1 gene in an orthotopic model

David I. Kleinerman, Colin P N Dinney, Wei Wei Zhang, Sue Hwa Lin, Nguyen T. Van, Jer Tsong Hsieh

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


Recently, we demonstrated that an immunoglobulin-like cell adhesion molecule, C-CAM, acts as a tumor suppressor in prostate cancer. It is known that C-CAM is expressed in many epithelial cell types. In this study, we tested the possibility that C-CAM may also suppress bladder cancer progression. We used an orthotopic tumor model, which provides a relevant organ condition for examining the interaction between primary tumor cells and their microenvironment; this interaction has a critical impact on the behavior of carcinoma. We constructed a recombinant adenovirus expressing C- CAM1 (an isoform of C-CAM) and infected the 253J B-V cell line, a tumorigenic human bladder carcinoma subline. In vitro, C-CAM1 protein was detected in C- CAM1 adenovirus-infected cells but not in antisense control virus-infected cells, and the levels of expression showed dose dependency. When these cells were injected orthotopically in nude mice, we found that the increased expression of C-CAM1 in the 253J B-V cells repressed the growth of 253J B-V- induced tumors. Taken together, these data indicate that C-CAM1 is a potent tumor suppressor in human bladder cancer.

Original languageEnglish (US)
Pages (from-to)3431-3435
Number of pages5
JournalCancer research
Issue number15
StatePublished - Aug 1 1996

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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