1H and 15N assignments and secondary structure of the Src SH3 domain

Hongtao Yu; Michael K. Rosen; Stuart L. Schreiber

doi:10.1016/0014-5793(93)81538-B

¹H and ¹⁵N assignments and secondary structure of the Src SH3 domain

Hongtao Yu, Michael K. Rosen, Stuart L. Schreiber

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

The ¹H and ¹⁵N sequential assignments of the Src SH3 domain have been determined through a combination of 2D and 3D Nuclear Magnetic Resonance (NMR) methods. The secondary structure of the protein has been identified based on long-range NOE patterns. The SH3 domain of Src consists largely of six β-strands that form two anti-parallel β-sheets.

Original language	English (US)
Pages (from-to)	87-92
Number of pages	6
Journal	FEBS Letters
Volume	324
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(93)81538-B
State	Published - Jun 7 1993

Keywords

Nuclear magnetic resonance
SH3 domain
Secondary structure
Src

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(93)81538-B

Cite this

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title = "1H and 15N assignments and secondary structure of the Src SH3 domain",

abstract = "The 1H and 15N sequential assignments of the Src SH3 domain have been determined through a combination of 2D and 3D Nuclear Magnetic Resonance (NMR) methods. The secondary structure of the protein has been identified based on long-range NOE patterns. The SH3 domain of Src consists largely of six β-strands that form two anti-parallel β-sheets.",

keywords = "Nuclear magnetic resonance, SH3 domain, Secondary structure, Src",

author = "Hongtao Yu and Rosen, {Michael K.} and Schreiber, {Stuart L.}",

note = "Funding Information: A~kno~c,le~genlenWtse thank C. Seidel-Dugan and J.S. Brugge (ARIAD Pharmaceuticals. Inc.) for providmg the Src SW3 domam cDNA: D.C Dalgarno for asststing m the acqutsttton of several NMR data sets. and Bruker Instruments for provtdmg instrument time This research was supported by fellowshtps from the American Chemtcal Society and Merck Sharp & Dohme Research Laboratortes (to M.K.R.) and grant from the National Instttute of General Medtcal Sciences (GM44993. to S.L S.).",

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T1 - 1H and 15N assignments and secondary structure of the Src SH3 domain

AU - Yu, Hongtao

AU - Rosen, Michael K.

AU - Schreiber, Stuart L.

N1 - Funding Information: A~kno~c,le~genlenWtse thank C. Seidel-Dugan and J.S. Brugge (ARIAD Pharmaceuticals. Inc.) for providmg the Src SW3 domam cDNA: D.C Dalgarno for asststing m the acqutsttton of several NMR data sets. and Bruker Instruments for provtdmg instrument time This research was supported by fellowshtps from the American Chemtcal Society and Merck Sharp & Dohme Research Laboratortes (to M.K.R.) and grant from the National Instttute of General Medtcal Sciences (GM44993. to S.L S.).

PY - 1993/6/7

Y1 - 1993/6/7

N2 - The 1H and 15N sequential assignments of the Src SH3 domain have been determined through a combination of 2D and 3D Nuclear Magnetic Resonance (NMR) methods. The secondary structure of the protein has been identified based on long-range NOE patterns. The SH3 domain of Src consists largely of six β-strands that form two anti-parallel β-sheets.

AB - The 1H and 15N sequential assignments of the Src SH3 domain have been determined through a combination of 2D and 3D Nuclear Magnetic Resonance (NMR) methods. The secondary structure of the protein has been identified based on long-range NOE patterns. The SH3 domain of Src consists largely of six β-strands that form two anti-parallel β-sheets.

KW - Nuclear magnetic resonance

KW - SH3 domain

KW - Secondary structure

KW - Src

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