Subdiffraction scattered light imaging of gold nanoparticles using structured illumination

Bo Jui Chang; Shiuan Huei Lin; Li Jun Chou; Su Yu Chiang

doi:10.1364/OL.36.004773

Subdiffraction scattered light imaging of gold nanoparticles using structured illumination

Bo Jui Chang, Shiuan Huei Lin, Li Jun Chou, Su Yu Chiang

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A reflective light-scattering (RLS) microscope with structured illumination (SI) provides subdiffraction resolution and improves the image quality of gold nanoparticles in biological systems. The three-dimensional (3D)-structured pattern is rapidly and precisely controlled with a spatial light modulator and scrambled at the conjugate image plane to increase spatial incoherence. The reconstructed SI-RLS image of 100nm gold nanoparticles reveals lateral and axial resolutions of approximately 117 and 428nm. We present a high-resolution image of gold nanoparticles inside a HeLa cell, with improved contrast.

Original language	English (US)
Pages (from-to)	4773-4775
Number of pages	3
Journal	Optics Letters
Volume	36
Issue number	24
DOIs	https://doi.org/10.1364/OL.36.004773
State	Published - Dec 15 2011
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.36.004773

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abstract = "A reflective light-scattering (RLS) microscope with structured illumination (SI) provides subdiffraction resolution and improves the image quality of gold nanoparticles in biological systems. The three-dimensional (3D)-structured pattern is rapidly and precisely controlled with a spatial light modulator and scrambled at the conjugate image plane to increase spatial incoherence. The reconstructed SI-RLS image of 100nm gold nanoparticles reveals lateral and axial resolutions of approximately 117 and 428nm. We present a high-resolution image of gold nanoparticles inside a HeLa cell, with improved contrast.",

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AU - Chang, Bo Jui

AU - Lin, Shiuan Huei

AU - Chou, Li Jun

AU - Chiang, Su Yu

PY - 2011/12/15

Y1 - 2011/12/15

N2 - A reflective light-scattering (RLS) microscope with structured illumination (SI) provides subdiffraction resolution and improves the image quality of gold nanoparticles in biological systems. The three-dimensional (3D)-structured pattern is rapidly and precisely controlled with a spatial light modulator and scrambled at the conjugate image plane to increase spatial incoherence. The reconstructed SI-RLS image of 100nm gold nanoparticles reveals lateral and axial resolutions of approximately 117 and 428nm. We present a high-resolution image of gold nanoparticles inside a HeLa cell, with improved contrast.

AB - A reflective light-scattering (RLS) microscope with structured illumination (SI) provides subdiffraction resolution and improves the image quality of gold nanoparticles in biological systems. The three-dimensional (3D)-structured pattern is rapidly and precisely controlled with a spatial light modulator and scrambled at the conjugate image plane to increase spatial incoherence. The reconstructed SI-RLS image of 100nm gold nanoparticles reveals lateral and axial resolutions of approximately 117 and 428nm. We present a high-resolution image of gold nanoparticles inside a HeLa cell, with improved contrast.

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JO - Optics Letters

JF - Optics Letters

IS - 24

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Subdiffraction scattered light imaging of gold nanoparticles using structured illumination

Abstract

ASJC Scopus subject areas

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