TY - JOUR
T1 - Structure of Escherichia coli RutC, a member of the YjgF family and putative aminoacrylate peracid reductase of the rut operon
AU - Knapik, Aleksandra Alicja
AU - Petkowski, Janusz Jurand
AU - Otwinowski, Zbyszek
AU - Cymborowski, Marcin Tadeusz
AU - Cooper, David Robert
AU - Chruszcz, Maksymilian
AU - Krajewska, Wanda Małgorzata
AU - Minor, Wladek
PY - 2012/11
Y1 - 2012/11
N2 - RutC is the third enzyme in the Escherichia coli rut pathway of uracil degradation. RutC belongs to the highly conserved YjgF family of proteins. The structure of the RutC protein was determined and refined to 1.95 Å resolution. The crystal belonged to space group P21212 and contained six molecules in the asymmetric unit. The structure was solved by SAD phasing and was refined to an R work of 19.3% (R free = 21.7%). The final model revealed that this protein has a Bacillus chorismate mutase-like fold and forms a homotrimer with a hydrophobic cavity in the center of the structure and ligand-binding clefts between two subunits. A likely function for RutC is the reduction of peroxy-aminoacrylate to aminoacrylate as a part of a detoxification process.
AB - RutC is the third enzyme in the Escherichia coli rut pathway of uracil degradation. RutC belongs to the highly conserved YjgF family of proteins. The structure of the RutC protein was determined and refined to 1.95 Å resolution. The crystal belonged to space group P21212 and contained six molecules in the asymmetric unit. The structure was solved by SAD phasing and was refined to an R work of 19.3% (R free = 21.7%). The final model revealed that this protein has a Bacillus chorismate mutase-like fold and forms a homotrimer with a hydrophobic cavity in the center of the structure and ligand-binding clefts between two subunits. A likely function for RutC is the reduction of peroxy-aminoacrylate to aminoacrylate as a part of a detoxification process.
KW - RutC
KW - YjgF superfamily
KW - aminoacrylate
KW - peroxy-aminoacrylate
KW - rut operon
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U2 - 10.1107/S1744309112041796
DO - 10.1107/S1744309112041796
M3 - Article
C2 - 23143235
AN - SCOPUS:84869022149
SN - 1744-3091
VL - 68
SP - 1294
EP - 1299
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 11
ER -