Structure of a Yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex

Christopher H.S. Aylett; Daniel Boehringer; Jan P. Erzberger; Tanja Schaefer; Nenad Ban

doi:10.1038/nsmb.2963

Structure of a Yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex

Christopher H.S. Aylett, Daniel Boehringer, Jan P. Erzberger, Tanja Schaefer, Nenad Ban

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.

Original language	English (US)
Pages (from-to)	269-271
Number of pages	3
Journal	Nature Structural and Molecular Biology
Volume	22
Issue number	3
DOIs	https://doi.org/10.1038/nsmb.2963
State	Published - Mar 6 2015

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Structural Biology
Molecular Biology

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AU - Schaefer, Tanja

AU - Ban, Nenad

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Structure of a Yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex

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