@article{525ee13b5b1846129d892d0ab02829db,
title = "Structural visualization of RNA polymerase III transcription machineries",
abstract = "RNA polymerase III (Pol III) transcription initiation requires the action of the transcription factor IIIB (TFIIIB) and is highly regulated. Here, we determine the structures of Pol III pre-initiation complexes (PICs) using single particle cryo-electron microscopy (cryo-EM). We observe stable Pol III–TFIIIB complexes using nucleic acid scaffolds mimicking various functional states, in which TFIIIB tightly encircles the upstream promoter DNA. There is an intricate interaction between TFIIIB and Pol III, which stabilizes the winged-helix domains of the C34 subunit of Pol III over the active site cleft. The architecture of Pol III PIC more resembles that of the Pol II PIC than the Pol I PIC. In addition, we also obtain a 3D reconstruction of Pol III in complex with TFIIIB using the elongation complex (EC) scaffold, shedding light on the mechanism of facilitated recycling of Pol III prior to transcription re-initiation.",
author = "Yan Han and Chunli Yan and Susan Fishbain and Ivaylo Ivanov and Yuan He",
note = "Funding Information: We thank Dr. Jonathan Remis for assistance with microscope operation and data collection and Jason Pattie for computer support. We are grateful to Ryan Abdella for helpful discussion and comments on the manuscript. We also thank the staff at the Structural Biology Facility (SBF) of Northwestern University for technical support. This work was supported by a Cornew Innovation Award from the Chemistry of Life Processes Institute at Northwestern University (to Y. He), a Catalyst Award by the Chicago Biomedical Consortium with support from the Searle Funds at The Chicago Community Trust (to Y. He), an Institutional Research Grant from the American Cancer Society (IRG-15-173-21 to Y. He), the National Institutes of Health grant GM110387 (to I. I.). Y. Han is a recipient of the Chicago Biomedical Consortium Postdoctoral Research Grant. Computational resources were provided in part by allocations from the National Science Foundation XSEDE program (CHE110042 to I. I.) and the National Energy Research Scientific Computing Center supported by the DOE Office of Science (contract DE-AC02-05CH11231). Publisher Copyright: {\textcopyright} 2018, The Author(s).",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41421-018-0044-z",
language = "English (US)",
volume = "4",
journal = "Cell Discovery",
issn = "2056-5968",
publisher = "Nature Publishing Group",
number = "1",
}