TY - JOUR
T1 - Structural studies of MAP Kinase cascade components.
AU - Goldsmith, Elizabeth J.
AU - Min, Xiaoshan
AU - He, Haixia
AU - Zhou, Tianjun
PY - 2010
Y1 - 2010
N2 - MAPK cascade components have been the subject of structural analysis, advancing our understanding of how these enzymes are activated and how they interact. A surprising finding has been that unique inactive conformers are adopted by many of these kinases. These inactive conformers are interesting and often require experimental phases to determine their crystal structures because molecular replacement techniques are not successful. Here, we describe the preparation of MAP2K MEK6 and MAP3K TAO2 substituted with selenomethionine (SeMet) for de novo phasing. TAO2 and SeMet TAO2 were expressed in insect cells.
AB - MAPK cascade components have been the subject of structural analysis, advancing our understanding of how these enzymes are activated and how they interact. A surprising finding has been that unique inactive conformers are adopted by many of these kinases. These inactive conformers are interesting and often require experimental phases to determine their crystal structures because molecular replacement techniques are not successful. Here, we describe the preparation of MAP2K MEK6 and MAP3K TAO2 substituted with selenomethionine (SeMet) for de novo phasing. TAO2 and SeMet TAO2 were expressed in insect cells.
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U2 - 10.1007/978-1-60761-795-2_13
DO - 10.1007/978-1-60761-795-2_13
M3 - Article
C2 - 20811986
AN - SCOPUS:79952111889
SN - 1064-3745
VL - 661
SP - 223
EP - 237
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -