We have characterized the cell surface IgG of LPS-stimulated B lymphocytes by surface iodination and specific immunoprecipitation. IgG3 was found to be the predominant IgG subclass expressed on the B cell surface after 5 days of stimulation with LPS. In contrast. IgG2b, IgG3, and IgG1 subclasses are all secreted. Cell surface IgG exhibits detergent binding characteristics not shown by secreted IgG and the y chains of the three subclasses of surface IgG examined have the apparent mol. wt of 64 Kd. some 9.000 larger than the H chains of secreted IgG.
ASJC Scopus subject areas
- Molecular Biology