TY - JOUR
T1 - Structural basis of transcription
T2 - Backtracked RNA Polymerase II at 3.4 Angstrom Resolution
AU - Wang, Dong
AU - Bushnell, David A.
AU - Huang, Xuhui
AU - Westover, Kenneth D.
AU - Levitt, Michael
AU - Kornberg, Roger D.
PY - 2009/5/29
Y1 - 2009/5/29
N2 - Transcribing RNA polymerases oscillate between three stable states, two of which, pre- and posttranslocated, were previously subjected to x-ray crystal structure determination. We report here the crystal structure of RNA polymerase II in the third state, the reverse translocated, or "backtracked" state. The defining feature of the backtracked structure is a binding site for the first backtracked nucleotide. This binding site is occupied in case of nucleotide misincorporation in the RNA or damage to the DNA, and is termed the "P"site because it supports proofreading. The predominant mechanism of proofreading is the excision of a dinucleotide in the presence of the elongation factor SII (TFIIS). Structure determination of a cocrystal with TFIIS reveals a rearrangement whereby cleavage of the RNA may take place.
AB - Transcribing RNA polymerases oscillate between three stable states, two of which, pre- and posttranslocated, were previously subjected to x-ray crystal structure determination. We report here the crystal structure of RNA polymerase II in the third state, the reverse translocated, or "backtracked" state. The defining feature of the backtracked structure is a binding site for the first backtracked nucleotide. This binding site is occupied in case of nucleotide misincorporation in the RNA or damage to the DNA, and is termed the "P"site because it supports proofreading. The predominant mechanism of proofreading is the excision of a dinucleotide in the presence of the elongation factor SII (TFIIS). Structure determination of a cocrystal with TFIIS reveals a rearrangement whereby cleavage of the RNA may take place.
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U2 - 10.1126/science.1168729
DO - 10.1126/science.1168729
M3 - Article
C2 - 19478184
AN - SCOPUS:66349138227
SN - 0036-8075
VL - 324
SP - 1203
EP - 1206
JO - Science
JF - Science
IS - 5931
ER -