Structural and functional analysis of 3′ untranslated region of mouse Period1 mRNA

Shihoko Kojima; Matsumi Hirose; Katsushi Tokunaga; Yoshiyuki Sakaki; Hajime Tei

doi:10.1016/S0006-291X(02)02938-8

Structural and functional analysis of 3′ untranslated region of mouse Period1 mRNA

Shihoko Kojima, Matsumi Hirose, Katsushi Tokunaga, Yoshiyuki Sakaki, Hajime Tei

Neuroscience

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

In order to investigate the post-transcriptional regulation of Period1 (Per1), the 3′-untranslated region (3′UTR) of mouse Per1 (mPer1) mRNA was characterized. In addition to high similarity between human and mouse Per1 3′UTRs, AU-rich element and differentiation control element were found in both species. Transient transfection assays using luc::mPer1 3′UTR fusion genes revealed that the mPer1 3′UTR repressed its own expression in a post-transcriptional manner. The region critical for this translational down-regulation was confined to nucleotide positions 322-517. These results suggest that the mPer1 3′UTR could be involved in the generation of time lag between the transcriptional and translational products of mPer1 in the suprachiasmatic nucleus.

Original language	English (US)
Pages (from-to)	1-7
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	301
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(02)02938-8
State	Published - Jan 31 2003

Keywords

3′-Untranslated region
Circadian rhythms
Mouse Period1 mRNA
Post-transcriptional regulation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(02)02938-8

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title = "Structural and functional analysis of 3′ untranslated region of mouse Period1 mRNA",

abstract = "In order to investigate the post-transcriptional regulation of Period1 (Per1), the 3′-untranslated region (3′UTR) of mouse Per1 (mPer1) mRNA was characterized. In addition to high similarity between human and mouse Per1 3′UTRs, AU-rich element and differentiation control element were found in both species. Transient transfection assays using luc::mPer1 3′UTR fusion genes revealed that the mPer1 3′UTR repressed its own expression in a post-transcriptional manner. The region critical for this translational down-regulation was confined to nucleotide positions 322-517. These results suggest that the mPer1 3′UTR could be involved in the generation of time lag between the transcriptional and translational products of mPer1 in the suprachiasmatic nucleus.",

keywords = "3′-Untranslated region, Circadian rhythms, Mouse Period1 mRNA, Post-transcriptional regulation",

author = "Shihoko Kojima and Matsumi Hirose and Katsushi Tokunaga and Yoshiyuki Sakaki and Hajime Tei",

note = "Funding Information: We thank Drs. Naoyuki Tsuchiya, Hirohiko Hohjoh, and Jun Ohashi for helpful discussions. This work was partially supported by research grants from the Japanese Ministry of Education, Science, Sports and Culture, and the Japanese Ministry of Health and Welfare.",

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AU - Kojima, Shihoko

AU - Hirose, Matsumi

AU - Tokunaga, Katsushi

AU - Sakaki, Yoshiyuki

AU - Tei, Hajime

N1 - Funding Information: We thank Drs. Naoyuki Tsuchiya, Hirohiko Hohjoh, and Jun Ohashi for helpful discussions. This work was partially supported by research grants from the Japanese Ministry of Education, Science, Sports and Culture, and the Japanese Ministry of Health and Welfare.

PY - 2003/1/31

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N2 - In order to investigate the post-transcriptional regulation of Period1 (Per1), the 3′-untranslated region (3′UTR) of mouse Per1 (mPer1) mRNA was characterized. In addition to high similarity between human and mouse Per1 3′UTRs, AU-rich element and differentiation control element were found in both species. Transient transfection assays using luc::mPer1 3′UTR fusion genes revealed that the mPer1 3′UTR repressed its own expression in a post-transcriptional manner. The region critical for this translational down-regulation was confined to nucleotide positions 322-517. These results suggest that the mPer1 3′UTR could be involved in the generation of time lag between the transcriptional and translational products of mPer1 in the suprachiasmatic nucleus.

AB - In order to investigate the post-transcriptional regulation of Period1 (Per1), the 3′-untranslated region (3′UTR) of mouse Per1 (mPer1) mRNA was characterized. In addition to high similarity between human and mouse Per1 3′UTRs, AU-rich element and differentiation control element were found in both species. Transient transfection assays using luc::mPer1 3′UTR fusion genes revealed that the mPer1 3′UTR repressed its own expression in a post-transcriptional manner. The region critical for this translational down-regulation was confined to nucleotide positions 322-517. These results suggest that the mPer1 3′UTR could be involved in the generation of time lag between the transcriptional and translational products of mPer1 in the suprachiasmatic nucleus.

KW - 3′-Untranslated region

KW - Circadian rhythms

KW - Mouse Period1 mRNA

KW - Post-transcriptional regulation

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Structural and functional analysis of 3′ untranslated region of mouse Period1 mRNA

Abstract

Keywords

ASJC Scopus subject areas

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