STING guides the STX17-SNAP29-VAMP8 complex assembly to control autophagy

Xiaoyu Song, Yufeng Xi, Ming Dai, Tao Li, Shihao Du, Yuxin Zhu, Mengjie Li, Yunze Li, Siqi Liu, Xia Ding, Xuebiao Yao, Ying Lai, Xing Liu

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The stimulator of interferon genes (STING) plays a pivotal role in orchestrating innate immunity, and dysregulated activity of STING has been implicated in the pathogenesis of autoimmune diseases. Recent findings suggest that bacterial infection activates STING, relieving ER stress, and triggers non-canonical autophagy by spatially regulating STX17. Despite these insights, the precise mechanism governing the dynamics of autophagosome fusion elicited by STING remains unclear. In this study, we demonstrate that dynamic STING activation guides the autophagy flux, mirroring the trajectory of canonical autophagy adaptors. STING engages in a physical interaction with STX17, and agonist-induced phosphorylation or degradation alleviates STING's inhibitory effects on the assembly of the STX17-SNAP29-VAMP8 complex. Consistent with these findings, degradation-deficient mutants hinder autophagy flux by impeding STX17-mediated autophagosome-lysosome fusion. Moreover, STING mutants associated with lupus disrupt the assembly of the STX17-SNAP29-VAMP8 complex and autophagy process, which lead to persistent STING activation and elevated IFN-β production. Our results highlight that the intracellular trajectory of STING, coupled with autophagy flux, guides the assembly and membrane fusion of the STX17-SNAP29-VAMP8 complex, ensuring the accurate regulation of innate immunity.

Original languageEnglish (US)
Article number100147
JournalCell Insight
Volume3
Issue number2
DOIs
StatePublished - Apr 2024
Externally publishedYes

Keywords

  • Autophagy
  • Innate immunity
  • Phosphorylation
  • STING
  • STX17

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Physiology

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