@article{9d71a605f3d344dcad838c5c854d00cb,
title = "Sterol-dependent nuclear import of ORP1S promotes LXR regulated trans-activation of apoE",
abstract = "Oxysterol binding protein related protein 1S (ORP1S) is a member of a family of sterol transport proteins. Here we present evidence that ORP1S translocates from the cytoplasm to the nucleus in response to sterol binding. The sterols that best promote nuclear import of ORP1S also activate the liver X receptor (LXR) transcription factors and we show that ORP1S binds to LXRs, promotes binding of LXRs to LXR response elements (LXREs) and specifically enhances LXR-dependent transcription via the ME.1 and ME.2 enhancer elements of the apoE gene. We propose that ORP1S is a cytoplasmic sterol sensor, which transports sterols to the nucleus and promotes LXR-dependent gene transcription through select enhancer elements.",
keywords = "LXR, NLS, Nuclear import, ORP1, ORP1S, Oxysterol",
author = "Sungsoo Lee and Wang, {Ping Yuan} and Yangsik Jeong and Mangelsdorf, {David J.} and Anderson, {Richard G W} and Peter Michaely",
note = "Funding Information: We would like to thank Jason Hall and Nupur Sharma for their valuable technical assistances, Brenda Pallares for administrative assistance and the U.T. Southwestern Live Cell Imaging Facility under the direction of Dr. Kate Luby-Phelps. This work was supported by grants from the National Institutes of Health , HL 85218 (PM), HL 20948 (RGWA), GM 52016 (RGWA), the Cecil H. Green Distinguished Chair in Cellular and Molecular Biology (RGWA), the Perot Family Foundation (RGWA), the Howard Hughes Medical Institute (DJM), the Robert A. Welch Foundation ( I-1275 ) (DJM) and Beatrice and Miguel Elias Distinguished Chair in Biomedical Science (DJM). DJM is an Investigator of the Howard Hughes Medical Institute. ",
year = "2012",
month = oct,
day = "1",
doi = "10.1016/j.yexcr.2012.06.012",
language = "English (US)",
volume = "318",
pages = "2128--2142",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "16",
}