TY - JOUR
T1 - Specific regional distribution of gec1 mRNAs in adult rat central nervous system
AU - Tolle, Fabrice
AU - Risold, Pierre Yves
AU - Mansuy-Schlick, Virginie
AU - Rossi, Emilie
AU - Boyer-Guittaut, Michaël
AU - Fraichard, Annick
AU - Jouvenot, Michèle
N1 - Funding Information:
This research work was supported by a fellowship from the Ministère de l'Enseignement Supérieur et de la Recherche (MESR) and by grants from MESR (E.A.3922) and from the Ligue Nationale Contre le Cancer (Comité du Doubs and Comité du Jura). We are particularly grateful to Dr. R. Olsen (Los Angeles, CA, USA) for the generous gift of GABARAP expression plasmid.
PY - 2008/5/19
Y1 - 2008/5/19
N2 - GEC1 protein shares high identity with GABARAP (GABAA Receptor-Associated Protein), interacts with tubulin and GABAA receptors and is potentially involved in intracellular transport processes. Recently, using quantitative real time PCR, we have reported the gec1 mRNA expression in different rat brain areas. In the present study, we investigated the cell types expressing gec1 in rat brain. Sense and anti-sense gec1 RNA probes, corresponding to the 3′-untranslated region, were generated. In northern blotting experiments, the anti-sense probe revealed only the 1.75 kb gec1 mRNAs. On the other hand, in immunohistochemistry experiments, GEC1 polyclonal antibodies did not discriminate between GEC1 and GABARAP proteins. Therefore, we used digoxigenin-labeled RNA probes for in situ hybridization (ISH) experiments to map the gec1 expression. Using the anti-sense probe, we detected the gec1 mRNAs specifically in neurons throughout the rostrocaudal extent of the brain as well as in the spinal cord. Although a majority of neurons expressed the gec1 mRNAs, different intensities of labeling were observed depending on the areas: the strongest labeling was observed in the isocortex, hippocampus, basal telencephalon, some thalamic and most of hypothalamic nuclei, cerebellum, and numerous brainstem nuclei. Furthermore, the gec1 mRNAs were intensely expressed in neurons involved in somatomotor and neuroendocrine functions and weakly expressed in sensory and reticular structures. These results corroborate the putative role of the GEC1 protein in the trafficking of receptor GABAA.
AB - GEC1 protein shares high identity with GABARAP (GABAA Receptor-Associated Protein), interacts with tubulin and GABAA receptors and is potentially involved in intracellular transport processes. Recently, using quantitative real time PCR, we have reported the gec1 mRNA expression in different rat brain areas. In the present study, we investigated the cell types expressing gec1 in rat brain. Sense and anti-sense gec1 RNA probes, corresponding to the 3′-untranslated region, were generated. In northern blotting experiments, the anti-sense probe revealed only the 1.75 kb gec1 mRNAs. On the other hand, in immunohistochemistry experiments, GEC1 polyclonal antibodies did not discriminate between GEC1 and GABARAP proteins. Therefore, we used digoxigenin-labeled RNA probes for in situ hybridization (ISH) experiments to map the gec1 expression. Using the anti-sense probe, we detected the gec1 mRNAs specifically in neurons throughout the rostrocaudal extent of the brain as well as in the spinal cord. Although a majority of neurons expressed the gec1 mRNAs, different intensities of labeling were observed depending on the areas: the strongest labeling was observed in the isocortex, hippocampus, basal telencephalon, some thalamic and most of hypothalamic nuclei, cerebellum, and numerous brainstem nuclei. Furthermore, the gec1 mRNAs were intensely expressed in neurons involved in somatomotor and neuroendocrine functions and weakly expressed in sensory and reticular structures. These results corroborate the putative role of the GEC1 protein in the trafficking of receptor GABAA.
KW - In situ hybridization
KW - Rat brain
KW - gabarap
KW - gec1
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U2 - 10.1016/j.brainres.2008.02.077
DO - 10.1016/j.brainres.2008.02.077
M3 - Article
C2 - 18423580
AN - SCOPUS:43049153253
SN - 0006-8993
VL - 1210
SP - 103
EP - 115
JO - Brain Research
JF - Brain Research
ER -