TY - JOUR
T1 - Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization
AU - Tskvitaria-Fuller, Irina
AU - Seth, Abhinav
AU - Mistry, Neeta
AU - Gu, Hua
AU - Rosen, Michael K.
AU - Wülfing, Christoph
PY - 2006/8/1
Y1 - 2006/8/1
N2 - T cell polarization toward and within the cellular interface with an APC is critical for effective T cell activation. The Rho family GTPase Cdc42 is a central regulator of cellular polarization. Using live-cell imaging, we characterized the spatiotemporal patterns of Cdc42 activity and their physiological regulation. Using three independent means of experimental manipulation of Cdc42 activity, we established that Cdc42 is a critical regulator of T cell actin dynamics, TCR clustering, and cell cycle entry. Using quantification of three-dimensional data, we could relate distinct spatiotemporal patterns of Cdc42 activity to specific elements of T cell activation. This result suggests that Cdc42 activity in specific locations at specific times is most critical for its function in T cell activation.
AB - T cell polarization toward and within the cellular interface with an APC is critical for effective T cell activation. The Rho family GTPase Cdc42 is a central regulator of cellular polarization. Using live-cell imaging, we characterized the spatiotemporal patterns of Cdc42 activity and their physiological regulation. Using three independent means of experimental manipulation of Cdc42 activity, we established that Cdc42 is a critical regulator of T cell actin dynamics, TCR clustering, and cell cycle entry. Using quantification of three-dimensional data, we could relate distinct spatiotemporal patterns of Cdc42 activity to specific elements of T cell activation. This result suggests that Cdc42 activity in specific locations at specific times is most critical for its function in T cell activation.
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U2 - 10.4049/jimmunol.177.3.1708
DO - 10.4049/jimmunol.177.3.1708
M3 - Article
C2 - 16849480
AN - SCOPUS:33746215226
SN - 0022-1767
VL - 177
SP - 1708
EP - 1720
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -