Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors

Katherine Luby-Phelps; Joseph Fogerty; Sheila A. Baker; Gregory J. Pazour; Joseph C. Besharse

doi:10.1016/j.visres.2007.08.022

Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors

Katherine Luby-Phelps, Joseph Fogerty, Sheila A. Baker, Gregory J. Pazour, Joseph C. Besharse

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Intraflagellar transport (IFT) of a ∼17S particle containing at least 16 distinct polypeptides is required for the assembly and maintenance of cilia and flagella. Although both genetic and biochemical evidence suggest a role for IFT in vertebrate photoreceptors, the spatial distribution of IFT proteins within photoreceptors remains poorly defined. We have evaluated the distribution of 4 IFT proteins using a combination of immunocytochemistry and rod-specific overexpression of GFP tagged IFT proteins. Endogenous IFT proteins are most highly concentrated within the inner segment, around the basal body, and within the outer segment IFT proteins are localized in discrete particles along the entire length of the axoneme. IFT52-GFP and IFT57-GFP mimicked this pattern in transgenic Xenopus.

Original language	English (US)
Pages (from-to)	413-423
Number of pages	11
Journal	Vision Research
Volume	48
Issue number	3
DOIs	https://doi.org/10.1016/j.visres.2007.08.022
State	Published - Feb 2008

Keywords

Connecting cilium
Intraflagellar transport
Kinesin
Microtubule
Photoreceptor
Rhodopsin

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

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10.1016/j.visres.2007.08.022

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title = "Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors",

abstract = "Intraflagellar transport (IFT) of a ∼17S particle containing at least 16 distinct polypeptides is required for the assembly and maintenance of cilia and flagella. Although both genetic and biochemical evidence suggest a role for IFT in vertebrate photoreceptors, the spatial distribution of IFT proteins within photoreceptors remains poorly defined. We have evaluated the distribution of 4 IFT proteins using a combination of immunocytochemistry and rod-specific overexpression of GFP tagged IFT proteins. Endogenous IFT proteins are most highly concentrated within the inner segment, around the basal body, and within the outer segment IFT proteins are localized in discrete particles along the entire length of the axoneme. IFT52-GFP and IFT57-GFP mimicked this pattern in transgenic Xenopus.",

keywords = "Connecting cilium, Intraflagellar transport, Kinesin, Microtubule, Photoreceptor, Rhodopsin",

author = "Katherine Luby-Phelps and Joseph Fogerty and Baker, {Sheila A.} and Pazour, {Gregory J.} and Besharse, {Joseph C.}",

note = "Funding Information: We are very grateful to Dr. Gregory Ning from the Medical College of Wisconsin EM Facility for assistance with the electron microscopy. Some of the data in this report were presented at the 2002 annual meeting of the American Society for Cell Biology of Cell Biology and the 2003 annual meeting of the Association for Research in Vision and Ophthalmology. This research was supported by grants from the National Science Foundation (NSF MCB 9604594, KLP) and the National Institutes of Health (NIH EY03222, JCB). ",

year = "2008",

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doi = "10.1016/j.visres.2007.08.022",

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AU - Luby-Phelps, Katherine

AU - Fogerty, Joseph

AU - Baker, Sheila A.

AU - Pazour, Gregory J.

AU - Besharse, Joseph C.

N1 - Funding Information: We are very grateful to Dr. Gregory Ning from the Medical College of Wisconsin EM Facility for assistance with the electron microscopy. Some of the data in this report were presented at the 2002 annual meeting of the American Society for Cell Biology of Cell Biology and the 2003 annual meeting of the Association for Research in Vision and Ophthalmology. This research was supported by grants from the National Science Foundation (NSF MCB 9604594, KLP) and the National Institutes of Health (NIH EY03222, JCB).

PY - 2008/2

Y1 - 2008/2

N2 - Intraflagellar transport (IFT) of a ∼17S particle containing at least 16 distinct polypeptides is required for the assembly and maintenance of cilia and flagella. Although both genetic and biochemical evidence suggest a role for IFT in vertebrate photoreceptors, the spatial distribution of IFT proteins within photoreceptors remains poorly defined. We have evaluated the distribution of 4 IFT proteins using a combination of immunocytochemistry and rod-specific overexpression of GFP tagged IFT proteins. Endogenous IFT proteins are most highly concentrated within the inner segment, around the basal body, and within the outer segment IFT proteins are localized in discrete particles along the entire length of the axoneme. IFT52-GFP and IFT57-GFP mimicked this pattern in transgenic Xenopus.

AB - Intraflagellar transport (IFT) of a ∼17S particle containing at least 16 distinct polypeptides is required for the assembly and maintenance of cilia and flagella. Although both genetic and biochemical evidence suggest a role for IFT in vertebrate photoreceptors, the spatial distribution of IFT proteins within photoreceptors remains poorly defined. We have evaluated the distribution of 4 IFT proteins using a combination of immunocytochemistry and rod-specific overexpression of GFP tagged IFT proteins. Endogenous IFT proteins are most highly concentrated within the inner segment, around the basal body, and within the outer segment IFT proteins are localized in discrete particles along the entire length of the axoneme. IFT52-GFP and IFT57-GFP mimicked this pattern in transgenic Xenopus.

KW - Connecting cilium

KW - Intraflagellar transport

KW - Kinesin

KW - Microtubule

KW - Photoreceptor

KW - Rhodopsin

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Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors

Abstract

Keywords

ASJC Scopus subject areas

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