Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke

Xuan Li; Jingjun Lyu; Ran Li; Vaibhav Jain; Yuntian Shen; Ángela del Águila; Ulrike Hoffmann; Huaxin Sheng; Wei Yang

doi:10.1186/s12974-022-02447-5

Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke

Xuan Li, Jingjun Lyu, Ran Li, Vaibhav Jain, Yuntian Shen, Ángela del Águila, Ulrike Hoffmann, Huaxin Sheng, Wei Yang

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Background: Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in the post-stroke brain constitutes a key component of stroke pathophysiology. This study aimed to determine how stroke affects immune cell populations in the aged brain based on molecular profiles of individual cells. Methods: Single-cell RNA sequencing and a new transient ischemic stroke mouse model with late reperfusion were used. Results: We generated, for the first time, a composite picture of immune cell populations in the stroke aged brain at single-cell resolution. We discovered at least 6 microglial subsets in the stroke aged brain, including a potentially stroke-specific subtype. Moreover, we identified major cell subpopulations formed by infiltrated myeloid cells after stroke, and revealed their unique molecular profiles. Conclusions: This study provided the first scRNA-seq data set for immune cells in the stroke aged brain, and offered novel insights into post-stroke immune cell heterogeneity.

Original language	English (US)
Article number	83
Journal	Journal of Neuroinflammation
Volume	19
Issue number	1
DOIs	https://doi.org/10.1186/s12974-022-02447-5
State	Published - Dec 2022
Externally published	Yes

Keywords

Dendritic cell
Late reperfusion
Microglia
Monocyte
Neuroinflammation
Neutrophil
Transcriptome
scRNA-seq

ASJC Scopus subject areas

Neuroscience(all)
Immunology
Neurology
Cellular and Molecular Neuroscience

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10.1186/s12974-022-02447-5

Cite this

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title = "Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke",

abstract = "Background: Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in the post-stroke brain constitutes a key component of stroke pathophysiology. This study aimed to determine how stroke affects immune cell populations in the aged brain based on molecular profiles of individual cells. Methods: Single-cell RNA sequencing and a new transient ischemic stroke mouse model with late reperfusion were used. Results: We generated, for the first time, a composite picture of immune cell populations in the stroke aged brain at single-cell resolution. We discovered at least 6 microglial subsets in the stroke aged brain, including a potentially stroke-specific subtype. Moreover, we identified major cell subpopulations formed by infiltrated myeloid cells after stroke, and revealed their unique molecular profiles. Conclusions: This study provided the first scRNA-seq data set for immune cells in the stroke aged brain, and offered novel insights into post-stroke immune cell heterogeneity.",

keywords = "Dendritic cell, Late reperfusion, Microglia, Monocyte, Neuroinflammation, Neutrophil, Transcriptome, scRNA-seq",

author = "Xuan Li and Jingjun Lyu and Ran Li and Vaibhav Jain and Yuntian Shen and {del {\'A}guila}, {\'A}ngela and Ulrike Hoffmann and Huaxin Sheng and Wei Yang",

note = "Funding Information: This study was supported by funds from the Department of Anesthesiology (Duke University Medical Center), and NIH grants R01NS099590 and R01HL157354. Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1186/s12974-022-02447-5",

language = "English (US)",

volume = "19",

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T1 - Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke

AU - Li, Xuan

AU - Lyu, Jingjun

AU - Li, Ran

AU - Jain, Vaibhav

AU - Shen, Yuntian

AU - del Águila, Ángela

AU - Hoffmann, Ulrike

AU - Sheng, Huaxin

AU - Yang, Wei

N1 - Funding Information: This study was supported by funds from the Department of Anesthesiology (Duke University Medical Center), and NIH grants R01NS099590 and R01HL157354. Publisher Copyright: © 2022, The Author(s).

PY - 2022/12

Y1 - 2022/12

N2 - Background: Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in the post-stroke brain constitutes a key component of stroke pathophysiology. This study aimed to determine how stroke affects immune cell populations in the aged brain based on molecular profiles of individual cells. Methods: Single-cell RNA sequencing and a new transient ischemic stroke mouse model with late reperfusion were used. Results: We generated, for the first time, a composite picture of immune cell populations in the stroke aged brain at single-cell resolution. We discovered at least 6 microglial subsets in the stroke aged brain, including a potentially stroke-specific subtype. Moreover, we identified major cell subpopulations formed by infiltrated myeloid cells after stroke, and revealed their unique molecular profiles. Conclusions: This study provided the first scRNA-seq data set for immune cells in the stroke aged brain, and offered novel insights into post-stroke immune cell heterogeneity.

AB - Background: Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in the post-stroke brain constitutes a key component of stroke pathophysiology. This study aimed to determine how stroke affects immune cell populations in the aged brain based on molecular profiles of individual cells. Methods: Single-cell RNA sequencing and a new transient ischemic stroke mouse model with late reperfusion were used. Results: We generated, for the first time, a composite picture of immune cell populations in the stroke aged brain at single-cell resolution. We discovered at least 6 microglial subsets in the stroke aged brain, including a potentially stroke-specific subtype. Moreover, we identified major cell subpopulations formed by infiltrated myeloid cells after stroke, and revealed their unique molecular profiles. Conclusions: This study provided the first scRNA-seq data set for immune cells in the stroke aged brain, and offered novel insights into post-stroke immune cell heterogeneity.

KW - Dendritic cell

KW - Late reperfusion

KW - Microglia

KW - Monocyte

KW - Neuroinflammation

KW - Neutrophil

KW - Transcriptome

KW - scRNA-seq

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Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke

Abstract

Keywords

ASJC Scopus subject areas

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