Single-cell analysis shows that adipose tissue of persons with both HIV and diabetes is enriched for clonal, cytotoxic, and CMV-specific CD4+ T cells

Celestine N. Wanjalla, Wyatt J. McDonnell, Ramesh Ram, Abha Chopra, Rama Gangula, Shay Leary, Mona Mashayekhi, Joshua D. Simmons, Christian M. Warren, Samuel Bailin, Curtis L. Gabriel, Liang Guo, Briana D. Furch, Morgan C. Lima, Beverly O. Woodward, La Toya Hannah, Mark A. Pilkinton, Daniela T. Fuller, Kenji Kawai, Renu VirmaniAloke V. Finn, Alyssa H. Hasty, Simon A. Mallal, Spyros A. Kalams, John R. Koethe

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Persons with HIV are at increased risk for diabetes mellitus compared with individuals without HIV. Adipose tissue is an important regulator of glucose and lipid metabolism, and adipose tissue T cells modulate local inflammatory responses and, by extension, adipocyte function. Persons with HIV and diabetes have a high proportion of CX3CR1+ GPR56+ CD57+ (C-G-C+) CD4+ T cells in adipose tissue, a subset of which are cytomegalovirus specific, whereas individuals with diabetes but without HIV have predominantly CD69+ CD4+ T cells. Adipose tissue CD69+ and C-G-C+ CD4+ T cell subsets demonstrate higher receptor clonality compared with the same cells in blood, potentially reflecting antigen-driven expansion, but C-G-C+ CD4+ T cells have a more inflammatory and cytotoxic RNA transcriptome. Future studies will explore whether viral antigens have a role in recruitment and proliferation of pro-inflammatory C-G-C+ CD4+ T cells in adipose tissue of persons with HIV.

Original languageEnglish (US)
Article number100205
JournalCell Reports Medicine
Volume2
Issue number2
DOIs
StatePublished - Feb 16 2021
Externally publishedYes

Keywords

  • adipose tissue
  • CD4 T cell
  • CD57
  • CD69
  • CX3CR1
  • cytomegalovirus
  • diabetes
  • GPR56
  • HIV
  • single cell

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

Fingerprint

Dive into the research topics of 'Single-cell analysis shows that adipose tissue of persons with both HIV and diabetes is enriched for clonal, cytotoxic, and CMV-specific CD4+ T cells'. Together they form a unique fingerprint.

Cite this