@article{bbfd200113a046c9a9c40e76eb9c16e4,
title = "Single-Cell Analysis of Foxp1-Driven Mechanisms Essential for Striatal Development",
abstract = "The transcription factor FOXP1 is one of the top five genes associated with autism spectrum disorder and has conserved enriched expression in striatal spiny projection neurons (SPNs). Anderson et al. show at single-cell resolution that Foxp1 is critical for proper striatal development and functions within distinct striatal cell types in mice.",
keywords = "FOXP1, autism, neurodevelopment, single-cell RNA sequencing, striatum",
author = "Anderson, {Ashley G.} and Ashwinikumar Kulkarni and Matthew Harper and Genevieve Konopka",
note = "Funding Information: Our sincerest thanks to Dr. Helen Lai, Dr. Jane Johnson, Dr. Said Kourrich, Dr. Marissa Co, and Dr. Fatma Ayhan for providing critical feedback on the manuscript. We thank Dr. Denise Ramirez, Dr. Julian Meeks, and Apoorva Ajy from the UT Southwestern Whole Brain Microscopy Facility (WBMF) for assistance with volumetric whole-brain imaging and automated image analysis. The WBMF is supported by the Texas Institute of Brain Injury and Repair and the UTSW Peter O{\textquoteright}Donnell, Jr. Brain Institute . We thank the Neuroscience Microscopy Facility, supported by the UT Southwestern Neuroscience Department and the UTSW Peter O{\textquoteright}Donnell, Jr. Brain Institute. We would also like to thank Dr. Shari Birnbaum at the UTSW Rodent Behavior Core for help performing the fear conditioning, novel-cage activity, and digigait analyses. G.K. is a Jon Heighten Scholar in Autism Research at UT Southwestern . This work was supported by the NIH / NIMH (grant T32-MH076690 to A.G.A.), the Simons Foundation (SFARI 573689 and 401220 ), the James S. McDonnell Foundation 21st Century Science Initiative in Understanding Human Cognition (Scholar Award 220020467 ), the Chan Zuckerberg Initiative , an advised fund of the Silicon Valley Community Foundation ( HCA-A-1704-01747 ), and the NIH (grants DC014702 , DC016340 , and MH102603 to G.K.). Funding Information: Our sincerest thanks to Dr. Helen Lai, Dr. Jane Johnson, Dr. Said Kourrich, Dr. Marissa Co, and Dr. Fatma Ayhan for providing critical feedback on the manuscript. We thank Dr. Denise Ramirez, Dr. Julian Meeks, and Apoorva Ajy from the UT Southwestern Whole Brain Microscopy Facility (WBMF) for assistance with volumetric whole-brain imaging and automated image analysis. The WBMF is supported by the Texas Institute of Brain Injury and Repair and the UTSW Peter O'Donnell, Jr. Brain Institute. We thank the Neuroscience Microscopy Facility, supported by the UT Southwestern Neuroscience Department and the UTSW Peter O'Donnell, Jr. Brain Institute. We would also like to thank Dr. Shari Birnbaum at the UTSW Rodent Behavior Core for help performing the fear conditioning, novel-cage activity, and digigait analyses. G.K. is a Jon Heighten Scholar in Autism Research at UT Southwestern. This work was supported by the NIH/NIMH (grant T32-MH076690 to A.G.A.), the Simons Foundation (SFARI 573689 and 401220), the James S. McDonnell Foundation 21st Century Science Initiative in Understanding Human Cognition (Scholar Award 220020467), the Chan Zuckerberg Initiative, an advised fund of the Silicon Valley Community Foundation (HCA-A-1704-01747), and the NIH (grants DC014702, DC016340, and MH102603 to G.K.). A.G.A. and G.K. designed the study and wrote the paper. A.G.A. performed all single-cell sequencing experiments and library preparations. A.A. performed all RNA/protein quantification analyses, IHC experiments, and mouse behavior experiments. A.K. developed the manual pipeline for scRNA-seq analysis. A.G.A. and A.K. analyzed the data. M.H. contributed to mouse husbandry and genotyping. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = mar,
day = "3",
doi = "10.1016/j.celrep.2020.02.030",
language = "English (US)",
volume = "30",
pages = "3051--3066.e7",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "9",
}