Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation

Sora Lee; Brian T. Craig; Carmelle V. Romain; Jingbo Qiao; Dai H. Chung

doi:10.1016/j.canlet.2014.08.033

Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation

Sora Lee, Brian T. Craig, Carmelle V. Romain, Jingbo Qiao, Dai H. Chung

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Cell division cycle 42 (CDC42), a small GTPase of the Rho-subfamily, regulates diverse cellular functions including proliferation, cytoskeletal rearrangement and even promotes malignant transformation. Here, we found that increased expression of CDC42 correlated with undifferentiated neuroblastoma as compared to a more benign phenotype. CDC42 inhibition decreased cell growth and soft agar colony formation, and increased cell death in BE(2)-C and BE(2)-M17 cell lines, but not in SK-N-AS. In addition, silencing of CDC42 decreased expression of N-myc in BE(2)-C and BE(2)-M17 cells. Our findings suggest that CDC42 may play a role in the regulation of aggressive neuroblastoma behavior.

Original language	English (US)
Pages (from-to)	210-216
Number of pages	7
Journal	Cancer Letters
Volume	355
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2014.08.033
State	Published - Dec 28 2014
Externally published	Yes

Keywords

AKT2
CDC42
N-myc
Neuroblastoma
Transformation
Twist

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/j.canlet.2014.08.033

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title = "Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation",

abstract = "Cell division cycle 42 (CDC42), a small GTPase of the Rho-subfamily, regulates diverse cellular functions including proliferation, cytoskeletal rearrangement and even promotes malignant transformation. Here, we found that increased expression of CDC42 correlated with undifferentiated neuroblastoma as compared to a more benign phenotype. CDC42 inhibition decreased cell growth and soft agar colony formation, and increased cell death in BE(2)-C and BE(2)-M17 cell lines, but not in SK-N-AS. In addition, silencing of CDC42 decreased expression of N-myc in BE(2)-C and BE(2)-M17 cells. Our findings suggest that CDC42 may play a role in the regulation of aggressive neuroblastoma behavior.",

keywords = "AKT2, CDC42, N-myc, Neuroblastoma, Transformation, Twist",

author = "Sora Lee and Craig, {Brian T.} and Romain, {Carmelle V.} and Jingbo Qiao and Chung, {Dai H.}",

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T1 - Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation

AU - Lee, Sora

AU - Craig, Brian T.

AU - Romain, Carmelle V.

AU - Qiao, Jingbo

AU - Chung, Dai H.

PY - 2014/12/28

Y1 - 2014/12/28

N2 - Cell division cycle 42 (CDC42), a small GTPase of the Rho-subfamily, regulates diverse cellular functions including proliferation, cytoskeletal rearrangement and even promotes malignant transformation. Here, we found that increased expression of CDC42 correlated with undifferentiated neuroblastoma as compared to a more benign phenotype. CDC42 inhibition decreased cell growth and soft agar colony formation, and increased cell death in BE(2)-C and BE(2)-M17 cell lines, but not in SK-N-AS. In addition, silencing of CDC42 decreased expression of N-myc in BE(2)-C and BE(2)-M17 cells. Our findings suggest that CDC42 may play a role in the regulation of aggressive neuroblastoma behavior.

AB - Cell division cycle 42 (CDC42), a small GTPase of the Rho-subfamily, regulates diverse cellular functions including proliferation, cytoskeletal rearrangement and even promotes malignant transformation. Here, we found that increased expression of CDC42 correlated with undifferentiated neuroblastoma as compared to a more benign phenotype. CDC42 inhibition decreased cell growth and soft agar colony formation, and increased cell death in BE(2)-C and BE(2)-M17 cell lines, but not in SK-N-AS. In addition, silencing of CDC42 decreased expression of N-myc in BE(2)-C and BE(2)-M17 cells. Our findings suggest that CDC42 may play a role in the regulation of aggressive neuroblastoma behavior.

KW - AKT2

KW - CDC42

KW - N-myc

KW - Neuroblastoma

KW - Transformation

KW - Twist

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Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation

Abstract

Keywords

ASJC Scopus subject areas

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