TY - JOUR
T1 - Short-term effect of induced alterations in testosterone levels on fasting plasma amino acid levels in healthy young men
AU - Barbara Sahlin, K.
AU - Pla, Indira
AU - de Siqueira Guedes, Jéssica
AU - Pawłowski, Krzysztof
AU - Appelqvist, Roger
AU - Marko-Varga, György
AU - Domont, Gilberto Barbosa
AU - Nogueira, Fábio César Sousa
AU - Giwercman, Aleksander
AU - Sanchez, Aniel
AU - Malm, Johan
N1 - Funding Information:
Funding: This research was funded by the Interreg V EU program, ReproUnion 2.0 20201846.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - Long term effect of testosterone (T) deficiency impairs metabolism and is associated with muscle degradation and metabolic disease. The association seems to have a bidirectional nature and is not well understood. The present study aims to investigate the early and unidirectional metabolic effect of induced T changes by measuring fasting amino acid (AA) levels in a human model, in which short-term T alterations were induced. We designed a human model of 30 healthy young males with pharmacologically induced T changes, which resulted in three time points for blood collection: (A) baseline, (B) low T (3 weeks post administration of gonadotropin releasing hormone antagonist) and (C) restored T (2 weeks after injection of T undecanoate). The influence of T on AAs was analyzed by spectrophotometry on plasma samples. Levels of 9 out of 23 AAs, of which 7 were essential AAs, were significantly increased at low T and are restored upon T supplementation. Levels of tyrosine and phenylalanine were most strongly associated to T changes. Short-term effect of T changes suggests an increased protein breakdown that is restored upon T supplementation. Fasting AA levels are able to monitor the early metabolic changes induced by the T fluctuations.
AB - Long term effect of testosterone (T) deficiency impairs metabolism and is associated with muscle degradation and metabolic disease. The association seems to have a bidirectional nature and is not well understood. The present study aims to investigate the early and unidirectional metabolic effect of induced T changes by measuring fasting amino acid (AA) levels in a human model, in which short-term T alterations were induced. We designed a human model of 30 healthy young males with pharmacologically induced T changes, which resulted in three time points for blood collection: (A) baseline, (B) low T (3 weeks post administration of gonadotropin releasing hormone antagonist) and (C) restored T (2 weeks after injection of T undecanoate). The influence of T on AAs was analyzed by spectrophotometry on plasma samples. Levels of 9 out of 23 AAs, of which 7 were essential AAs, were significantly increased at low T and are restored upon T supplementation. Levels of tyrosine and phenylalanine were most strongly associated to T changes. Short-term effect of T changes suggests an increased protein breakdown that is restored upon T supplementation. Fasting AA levels are able to monitor the early metabolic changes induced by the T fluctuations.
KW - Gluconeogenesis
KW - Protein breakdown
KW - Testosterone
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U2 - 10.3390/life11111276
DO - 10.3390/life11111276
M3 - Article
C2 - 34833152
AN - SCOPUS:85120061392
SN - 0024-3019
VL - 11
JO - Life
JF - Life
IS - 11
M1 - 1276
ER -