Sequestration of the G protein βγ subunit complex inhibits receptor-mediated endocytosis

Chieh Lin Hsin Chieh Lin, J. A. Duncan, T. Kozasa, A. G. Gilman

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


Cell surface receptors that mediate endocytosis cluster into clathrin-coated pits, which pinch off to form vesicles that transport the receptors and their ligands. This multi-step process requires the coordinated action of many factors, including GTP-hydrolyzing proteins such as dynamin and regulators of actin cytoskeleton assembly. We note herein that sequestration of heterotrimeric G protein βγ subunits in intact cells strongly inhibits clathrin-coated pit-mediated endocytosis and causes rearrangement of the actin cytoskeleton. Our results suggest that cells contain a pool of free βγ and that it functions constitutively to permit endocytosis.

Original languageEnglish (US)
Pages (from-to)5057-5060
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number9
StatePublished - Apr 28 1998

ASJC Scopus subject areas

  • General


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