Sequencing of cDNA inserts encoding aromatase cytochrome P-450 (P-450AROM)

Evan R. Simpson; Claudia T. Evans; C. Jo Corbin; Frances E. Powell; Diana B. Ledesma; Carole R. Mendelson

doi:10.1016/0303-7207(87)90054-2

Sequencing of cDNA inserts encoding aromatase cytochrome P-450 (P-450_AROM)

Evan R. Simpson, Claudia T. Evans, C. Jo Corbin, Frances E. Powell, Diana B. Ledesma, Carole R. Mendelson

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Two cDNA inserts complementary to mRNA encoding aromatase cytochrome P-450 (P-450_AROM) have been isolated and characterized by restriction mapping and sequencing. The overlapping sequence encoded by these inserts is identical, and a putative heme-binding region has been identified. In addition, the open reading frame contains the sequences of all four cysteine-containing tryptic peptides isolated by Chen et al. (1986) from purified cytochrome P-450_AROM. The inserts differ in the use of alternative poly A-addition signals, which is consistent with the presence of two major species of mRNA in human placenta, of 3.0 and 2.4 kb, which hybridize to these inserts. The identity of sequence between the two inserts and the likely presence of alternative poly A-addition signals, is suggestive that only one form of cytochrome P-450_AROM is encoded by these mRNA species.

Original language	English (US)
Pages (from-to)	267-272
Number of pages	6
Journal	Molecular and Cellular Endocrinology
Volume	52
Issue number	3
DOIs	https://doi.org/10.1016/0303-7207(87)90054-2
State	Published - Aug 1987

Keywords

(Sequencing)
Cytochrome P-450
cDNA insert

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

Access to Document

10.1016/0303-7207(87)90054-2

Cite this

@article{81365300f084406cbd32e22d23d275c1,

title = "Sequencing of cDNA inserts encoding aromatase cytochrome P-450 (P-450AROM)",

abstract = "Two cDNA inserts complementary to mRNA encoding aromatase cytochrome P-450 (P-450AROM) have been isolated and characterized by restriction mapping and sequencing. The overlapping sequence encoded by these inserts is identical, and a putative heme-binding region has been identified. In addition, the open reading frame contains the sequences of all four cysteine-containing tryptic peptides isolated by Chen et al. (1986) from purified cytochrome P-450AROM. The inserts differ in the use of alternative poly A-addition signals, which is consistent with the presence of two major species of mRNA in human placenta, of 3.0 and 2.4 kb, which hybridize to these inserts. The identity of sequence between the two inserts and the likely presence of alternative poly A-addition signals, is suggestive that only one form of cytochrome P-450AROM is encoded by these mRNA species.",

keywords = "(Sequencing), Cytochrome P-450, cDNA insert",

author = "Simpson, {Evan R.} and Evans, {Claudia T.} and Corbin, {C. Jo} and Powell, {Frances E.} and Ledesma, {Diana B.} and Mendelson, {Carole R.}",

note = "Funding Information: The authors gratefully acknowledget he help and advice of Dr. Michael McPhaul of the De-partmento f Medicine of this Institution, as well as his gift of the JM109 cells. We also acknowl-edget he skilled editorial assistanceo f Dolly Tut-ton. This work was supported,in part, by USPHS grantsN os. AM-31206 and AG-00306.C .T.E. was supportedi,n part, by USPHS Training Grant No. 5-T32-HDO7190.",

year = "1987",

month = aug,

doi = "10.1016/0303-7207(87)90054-2",

language = "English (US)",

volume = "52",

pages = "267--272",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

number = "3",

}

TY - JOUR

T1 - Sequencing of cDNA inserts encoding aromatase cytochrome P-450 (P-450AROM)

AU - Simpson, Evan R.

AU - Evans, Claudia T.

AU - Corbin, C. Jo

AU - Powell, Frances E.

AU - Ledesma, Diana B.

AU - Mendelson, Carole R.

N1 - Funding Information: The authors gratefully acknowledget he help and advice of Dr. Michael McPhaul of the De-partmento f Medicine of this Institution, as well as his gift of the JM109 cells. We also acknowl-edget he skilled editorial assistanceo f Dolly Tut-ton. This work was supported,in part, by USPHS grantsN os. AM-31206 and AG-00306.C .T.E. was supportedi,n part, by USPHS Training Grant No. 5-T32-HDO7190.

PY - 1987/8

Y1 - 1987/8

N2 - Two cDNA inserts complementary to mRNA encoding aromatase cytochrome P-450 (P-450AROM) have been isolated and characterized by restriction mapping and sequencing. The overlapping sequence encoded by these inserts is identical, and a putative heme-binding region has been identified. In addition, the open reading frame contains the sequences of all four cysteine-containing tryptic peptides isolated by Chen et al. (1986) from purified cytochrome P-450AROM. The inserts differ in the use of alternative poly A-addition signals, which is consistent with the presence of two major species of mRNA in human placenta, of 3.0 and 2.4 kb, which hybridize to these inserts. The identity of sequence between the two inserts and the likely presence of alternative poly A-addition signals, is suggestive that only one form of cytochrome P-450AROM is encoded by these mRNA species.

AB - Two cDNA inserts complementary to mRNA encoding aromatase cytochrome P-450 (P-450AROM) have been isolated and characterized by restriction mapping and sequencing. The overlapping sequence encoded by these inserts is identical, and a putative heme-binding region has been identified. In addition, the open reading frame contains the sequences of all four cysteine-containing tryptic peptides isolated by Chen et al. (1986) from purified cytochrome P-450AROM. The inserts differ in the use of alternative poly A-addition signals, which is consistent with the presence of two major species of mRNA in human placenta, of 3.0 and 2.4 kb, which hybridize to these inserts. The identity of sequence between the two inserts and the likely presence of alternative poly A-addition signals, is suggestive that only one form of cytochrome P-450AROM is encoded by these mRNA species.

KW - (Sequencing)

KW - Cytochrome P-450

KW - cDNA insert

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