TY - JOUR
T1 - Sequence-specific remodeling of a topologically complex RNP substrate by Spb4
AU - Cruz, Victor Emmanuel
AU - Sekulski, Kamil
AU - Peddada, Nagesh
AU - Sailer, Carolin
AU - Balasubramanian, Sahana
AU - Weirich, Christine S.
AU - Stengel, Florian
AU - Erzberger, Jan P.
N1 - Funding Information:
We thank M. Rosen, L. Rice, J. Berger and X. Bai for discussions and M. Parker for help with live-cell imaging. We thank D. Stoddard at the UTSW Cryo-Electron Microscopy Facility, funded in part by the CPRIT Core Facility Support Award RP170644, and J. Chen at the UTSW Structural Biology Lab. A portion of this research was supported by NIH grant U24GM129547 and performed at the PNCC at OHSU and accessed through EMSL (grid.436923.9), a DOE Office of Science User Facility sponsored by the Office of Biological and Environmental Research, with the assistance of T. Humphreys. F. S. was supported by funding from the German Research Foundation (STE 2517/1 and STE 2517/5-1) and its Collaborative Research Centre 969 (Project A06). J. P. E. was supported by the Cancer Prevention and Research Institute of Texas (RR150074), the Welch Foundation (I-1897), the UTSW Endowed Scholars Fund and the National Institutes of Health (GM135617-01).
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2022/12
Y1 - 2022/12
N2 - DEAD-box ATPases are ubiquitous enzymes essential in all aspects of RNA biology. However, the limited in vitro catalytic activities described for these enzymes are at odds with their complex cellular roles, most notably in driving large-scale RNA remodeling steps during the assembly of ribonucleoproteins (RNPs). We describe cryo-EM structures of 60S ribosomal biogenesis intermediates that reveal how context-specific RNA unwinding by the DEAD-box ATPase Spb4 results in extensive, sequence-specific remodeling of rRNA secondary structure. Multiple cis and trans interactions stabilize Spb4 in a post-catalytic, high-energy intermediate that drives the organization of the three-way junction at the base of rRNA domain IV. This mechanism explains how limited strand separation by DEAD-box ATPases is leveraged to provide non-equilibrium directionality and ensure efficient and accurate RNP assembly.
AB - DEAD-box ATPases are ubiquitous enzymes essential in all aspects of RNA biology. However, the limited in vitro catalytic activities described for these enzymes are at odds with their complex cellular roles, most notably in driving large-scale RNA remodeling steps during the assembly of ribonucleoproteins (RNPs). We describe cryo-EM structures of 60S ribosomal biogenesis intermediates that reveal how context-specific RNA unwinding by the DEAD-box ATPase Spb4 results in extensive, sequence-specific remodeling of rRNA secondary structure. Multiple cis and trans interactions stabilize Spb4 in a post-catalytic, high-energy intermediate that drives the organization of the three-way junction at the base of rRNA domain IV. This mechanism explains how limited strand separation by DEAD-box ATPases is leveraged to provide non-equilibrium directionality and ensure efficient and accurate RNP assembly.
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U2 - 10.1038/s41594-022-00874-9
DO - 10.1038/s41594-022-00874-9
M3 - Article
C2 - 36482249
AN - SCOPUS:85143604400
SN - 1545-9993
VL - 29
SP - 1228
EP - 1238
JO - Nature Structural Biology
JF - Nature Structural Biology
IS - 12
ER -