Abstract
A set of fifteen 2-naphthol-derived atropisomers were evaluated on three different cyclofructan-based chiral stationary phases (CSP). The cyclofructan CSPs were a dimethylphenyl-derivatized cyclofructan 7 (CF7-DMP), a isopropyl (CF6-P) and a R-naphthylethyl cyclofructan 6 (CF6-RN) derivative, all bonded to 5-µm spherical fully porous silica particles packed into three 25 cm × 4.6 mm columns (commercially available as Larihc columns). The 15 atropisomers were analyzed in the normal-phase mode with heptane/alcohol mobile phases. The CF7-DMP column was the most effective partially or fully separating 14 of the 15 compounds (93%). All 15 compounds could be separated by at least one cyclofructan column. Five compounds could be partially or fully separated by all three CSPs. The effect of ethanol, 2-propanol and butanol as 5 and 10% v/v polar modifiers in heptane was studied. A prototype 15 cm × 4.6 mm column packed with superficially porous 2.7 µm CF6-P bonded particles was tested with the same set of compounds and a standard HPLC system. The increased efficiency and solvent saving were significant.
Original language | English (US) |
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Pages (from-to) | 710-717 |
Number of pages | 8 |
Journal | Journal of Liquid Chromatography and Related Technologies |
Volume | 39 |
Issue number | 15 |
DOIs | |
State | Published - Sep 13 2016 |
Keywords
- 2-naphthol phenyl derivatives
- Atropisomer separation
- cyclofructan selectors
- superficially porous particles
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Pharmaceutical Science
- Clinical Biochemistry