TY - JOUR
T1 - Separable enhancer sequences regulate the expression of the neural bHLH transcription factor neurogenin 1
AU - Nakada, Yuji
AU - Parab, Preeti
AU - Simmons, Andrew
AU - Omer-Abdalla, Adil
AU - Johnson, Jane E.
N1 - Funding Information:
The original mouse ngn1 genomic clones were kindly provided by Q. Ma and D. Anderson. The zebrafish ngn1 genomic sequence was generously shared by P. Blader and U. Strähle before publication. We thank Drs. A. Helms and R. M. Henke for helpful comments on the manuscript. This work was supported in part by the March of Dimes Foundation and NIH HD37832.
PY - 2004/7/15
Y1 - 2004/7/15
N2 - Ngn1 is a basic helix-loop-helix (bHLH) transcription factor expressed in specific regions within the developing brain and spinal cord, sensory ganglia, and olfactory epithelium. We have identified sequences both 5′ and 3′ of the mouse ngn1 gene that function in regulating ngn1 expression, and each of these sequences contains distinct regulatory cassettes for different subregions of the expression domain. Enhancers for expression in ngn1 domains of the midbrain, hindbrain, trigeminal ganglia, and ventral-neural tube appear redundant and are spread both 5′ and 3′ of the ngn1 coding sequence. In contrast, a single discrete dorsal-neural tube enhancer was located in the 5′ sequence that is conserved among mouse, human, chick, and zebrafish ngn1 genes. Functionally, this enhancer is both necessary and sufficient for driving expression of a heterologous reporter in transgenic mice specifically to the dorsal domain of ngn1 expression in the spinal neural tube. Thus, sequences are identified that can be used to direct temporally and spatially restricted expression of heterologous genes to the developing neural tube.
AB - Ngn1 is a basic helix-loop-helix (bHLH) transcription factor expressed in specific regions within the developing brain and spinal cord, sensory ganglia, and olfactory epithelium. We have identified sequences both 5′ and 3′ of the mouse ngn1 gene that function in regulating ngn1 expression, and each of these sequences contains distinct regulatory cassettes for different subregions of the expression domain. Enhancers for expression in ngn1 domains of the midbrain, hindbrain, trigeminal ganglia, and ventral-neural tube appear redundant and are spread both 5′ and 3′ of the ngn1 coding sequence. In contrast, a single discrete dorsal-neural tube enhancer was located in the 5′ sequence that is conserved among mouse, human, chick, and zebrafish ngn1 genes. Functionally, this enhancer is both necessary and sufficient for driving expression of a heterologous reporter in transgenic mice specifically to the dorsal domain of ngn1 expression in the spinal neural tube. Thus, sequences are identified that can be used to direct temporally and spatially restricted expression of heterologous genes to the developing neural tube.
KW - Basic helix-loop-helix transcription factor
KW - Dorsal-neural tube
KW - Neurogenin 1
KW - Spinal cord development
KW - Transcriptional regulation
KW - Transgenic mice
KW - Ventral-neural tube
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U2 - 10.1016/j.ydbio.2004.04.021
DO - 10.1016/j.ydbio.2004.04.021
M3 - Article
C2 - 15223348
AN - SCOPUS:3042543027
SN - 0012-1606
VL - 271
SP - 479
EP - 487
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -