Sensitization of radioresistant prostate cancer cells by resveratrol isolated from arachis hypogaea stems

Yu An Chen; Hsiu Man Lien; Min Chuan Kao; U. Ging Lo; Li Chiung Lin; Chun Jung Lin; Sheau Jiun Chang; Chia Chang Chen; Jer Tsong Hsieh; Ho Lin; Chih Hsin Tang; Chih Ho Lai

doi:10.1371/journal.pone.0169204

Sensitization of radioresistant prostate cancer cells by resveratrol isolated from arachis hypogaea stems

Yu An Chen, Hsiu Man Lien, Min Chuan Kao, U. Ging Lo, Li Chiung Lin, Chun Jung Lin, Sheau Jiun Chang, Chia Chang Chen, Jer Tsong Hsieh, Ho Lin, Chih Hsin Tang, Chih Ho Lai

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Abstract

Resveratrol (RV, 3,4,5-trihydroxystilbene) is naturally produced by a wide variety of plants including grapes and peanuts (Arachis hypogaea). However, the yield of RV from peanut stem and its potential radiosensitizing effects in prostate cancer (PCa) have not been well investigated. In this study, we characterized RV in peanut stem extract (PSE) for the first time and showed that both RV and PSE dose-dependently induced cell death in DOC-2/DAB2 interactive protein (DAB2IP)-deficient PCa cells with the radioresistant phenotype. Furthermore, the combination of radiation with either RV or PSE induced the death of radioresistant PCa cells through delayed repair of radiation-induced DNA double-strand break (DSB) and prolonged G2/M arrest, which induced apoptosis. The administration of RV and PSE effectively enhanced radiation therapy in the shDAB2IP PCa xenograft mouse model. These results demonstrate the promising synergistic effect of RV and PSE combined with radiation in the treatment of radioresistant PCa.

Original language	English (US)
Article number	e0169204
Journal	PloS one
Volume	12
Issue number	1
DOIs	https://doi.org/10.1371/journal.pone.0169204
State	Published - Jan 2017

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title = "Sensitization of radioresistant prostate cancer cells by resveratrol isolated from arachis hypogaea stems",

abstract = "Resveratrol (RV, 3,4,5-trihydroxystilbene) is naturally produced by a wide variety of plants including grapes and peanuts (Arachis hypogaea). However, the yield of RV from peanut stem and its potential radiosensitizing effects in prostate cancer (PCa) have not been well investigated. In this study, we characterized RV in peanut stem extract (PSE) for the first time and showed that both RV and PSE dose-dependently induced cell death in DOC-2/DAB2 interactive protein (DAB2IP)-deficient PCa cells with the radioresistant phenotype. Furthermore, the combination of radiation with either RV or PSE induced the death of radioresistant PCa cells through delayed repair of radiation-induced DNA double-strand break (DSB) and prolonged G2/M arrest, which induced apoptosis. The administration of RV and PSE effectively enhanced radiation therapy in the shDAB2IP PCa xenograft mouse model. These results demonstrate the promising synergistic effect of RV and PSE combined with radiation in the treatment of radioresistant PCa.",

author = "Chen, {Yu An} and Lien, {Hsiu Man} and Kao, {Min Chuan} and Lo, {U. Ging} and Lin, {Li Chiung} and Lin, {Chun Jung} and Chang, {Sheau Jiun} and Chen, {Chia Chang} and Hsieh, {Jer Tsong} and Ho Lin and Tang, {Chih Hsin} and Lai, {Chih Ho}",

note = "Publisher Copyright: {\textcopyright} 2017 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2017",

month = jan,

doi = "10.1371/journal.pone.0169204",

language = "English (US)",

volume = "12",

journal = "PloS one",

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T1 - Sensitization of radioresistant prostate cancer cells by resveratrol isolated from arachis hypogaea stems

AU - Chen, Yu An

AU - Lien, Hsiu Man

AU - Kao, Min Chuan

AU - Lo, U. Ging

AU - Lin, Li Chiung

AU - Lin, Chun Jung

AU - Chang, Sheau Jiun

AU - Chen, Chia Chang

AU - Hsieh, Jer Tsong

AU - Lin, Ho

AU - Tang, Chih Hsin

AU - Lai, Chih Ho

N1 - Publisher Copyright: © 2017 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2017/1

Y1 - 2017/1

N2 - Resveratrol (RV, 3,4,5-trihydroxystilbene) is naturally produced by a wide variety of plants including grapes and peanuts (Arachis hypogaea). However, the yield of RV from peanut stem and its potential radiosensitizing effects in prostate cancer (PCa) have not been well investigated. In this study, we characterized RV in peanut stem extract (PSE) for the first time and showed that both RV and PSE dose-dependently induced cell death in DOC-2/DAB2 interactive protein (DAB2IP)-deficient PCa cells with the radioresistant phenotype. Furthermore, the combination of radiation with either RV or PSE induced the death of radioresistant PCa cells through delayed repair of radiation-induced DNA double-strand break (DSB) and prolonged G2/M arrest, which induced apoptosis. The administration of RV and PSE effectively enhanced radiation therapy in the shDAB2IP PCa xenograft mouse model. These results demonstrate the promising synergistic effect of RV and PSE combined with radiation in the treatment of radioresistant PCa.

AB - Resveratrol (RV, 3,4,5-trihydroxystilbene) is naturally produced by a wide variety of plants including grapes and peanuts (Arachis hypogaea). However, the yield of RV from peanut stem and its potential radiosensitizing effects in prostate cancer (PCa) have not been well investigated. In this study, we characterized RV in peanut stem extract (PSE) for the first time and showed that both RV and PSE dose-dependently induced cell death in DOC-2/DAB2 interactive protein (DAB2IP)-deficient PCa cells with the radioresistant phenotype. Furthermore, the combination of radiation with either RV or PSE induced the death of radioresistant PCa cells through delayed repair of radiation-induced DNA double-strand break (DSB) and prolonged G2/M arrest, which induced apoptosis. The administration of RV and PSE effectively enhanced radiation therapy in the shDAB2IP PCa xenograft mouse model. These results demonstrate the promising synergistic effect of RV and PSE combined with radiation in the treatment of radioresistant PCa.

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Sensitization of radioresistant prostate cancer cells by resveratrol isolated from arachis hypogaea stems

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