Abstract
The ribosome-inactivating proteins, bryodin, from Bryonia dioica, and momordin, from Momordica charantia, were coupled by a disulphide bond to a monoclonal anti-Thy 1.1 antibody (OX7). Both immunotoxins were specifically cytotoxic to the Thy 1.1-expressing mouse lymphoma cell* line AKR-A in vitro. The OX7-bryodin immunotoxins were the more powerfully toxic and reduced protein synthesis in AKRA cells by 50% at a concentration of 1-4 x 10‑11 M as compared with 1 x 10‑9 M for the OX7-momordin immunotoxins. Neither of the immunotoxins was toxic to mouse lymphoma EL4 cells, which lack the Thy 1.1 antigen, at concentrations up to 3 x 10‑8 M. Further, bryodin and momordin immunotoxins made from an antibody (RIO) of irrelevant specificity were without effect on AKR-A cells.
Original language | English (US) |
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Pages (from-to) | 558-561 |
Number of pages | 4 |
Journal | British journal of cancer |
Volume | 58 |
Issue number | 5 |
DOIs | |
State | Published - Nov 1988 |
ASJC Scopus subject areas
- Oncology
- Cancer Research