Screening and Detection of Apoptosis

Sergio Huerta; Emily J. Goulet; Sara Huerta-Yepez; Edward H. Livingston

doi:10.1016/j.jss.2006.07.034

Screening and Detection of Apoptosis

Sergio Huerta, Emily J. Goulet, Sara Huerta-Yepez, Edward H. Livingston

Research output: Contribution to journal › Review article › peer-review

187 Scopus citations

Abstract

Since programmed cell death was first described by the electron microscopic cellular changes demonstrating an organized form of cell death over 30 years ago, it has undergone a great deal of scrutiny as a potential target for several diseases including cancer. The techniques for the study of apoptosis have evolved accordingly. Methodologies for the study of apoptosis were examined by a MEDLINE search of the English-language literature and are summarized in this review. This review discusses the various ways to study apoptosis with specific assays, reagents, and molecules. The particular advantages and disadvantages of each method are reviewed.

Original language	English (US)
Pages (from-to)	143-156
Number of pages	14
Journal	Journal of Surgical Research
Volume	139
Issue number	1
DOIs	https://doi.org/10.1016/j.jss.2006.07.034
State	Published - May 1 2007

Keywords

AIF
Apaf-1
Bcl-2
caspases
death receptor pathway
proteosome

ASJC Scopus subject areas

Surgery

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10.1016/j.jss.2006.07.034

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title = "Screening and Detection of Apoptosis",

abstract = "Since programmed cell death was first described by the electron microscopic cellular changes demonstrating an organized form of cell death over 30 years ago, it has undergone a great deal of scrutiny as a potential target for several diseases including cancer. The techniques for the study of apoptosis have evolved accordingly. Methodologies for the study of apoptosis were examined by a MEDLINE search of the English-language literature and are summarized in this review. This review discusses the various ways to study apoptosis with specific assays, reagents, and molecules. The particular advantages and disadvantages of each method are reviewed.",

keywords = "AIF, Apaf-1, Bcl-2, caspases, death receptor pathway, proteosome",

author = "Sergio Huerta and Goulet, {Emily J.} and Sara Huerta-Yepez and Livingston, {Edward H.}",

note = "Funding Information: This work was supported by a grant from the Dallas Veterans Administration Research Fund and the Hudson-Penn Surgery Fund.",

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AU - Huerta, Sergio

AU - Goulet, Emily J.

AU - Huerta-Yepez, Sara

AU - Livingston, Edward H.

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N2 - Since programmed cell death was first described by the electron microscopic cellular changes demonstrating an organized form of cell death over 30 years ago, it has undergone a great deal of scrutiny as a potential target for several diseases including cancer. The techniques for the study of apoptosis have evolved accordingly. Methodologies for the study of apoptosis were examined by a MEDLINE search of the English-language literature and are summarized in this review. This review discusses the various ways to study apoptosis with specific assays, reagents, and molecules. The particular advantages and disadvantages of each method are reviewed.

AB - Since programmed cell death was first described by the electron microscopic cellular changes demonstrating an organized form of cell death over 30 years ago, it has undergone a great deal of scrutiny as a potential target for several diseases including cancer. The techniques for the study of apoptosis have evolved accordingly. Methodologies for the study of apoptosis were examined by a MEDLINE search of the English-language literature and are summarized in this review. This review discusses the various ways to study apoptosis with specific assays, reagents, and molecules. The particular advantages and disadvantages of each method are reviewed.

KW - AIF

KW - Apaf-1

KW - Bcl-2

KW - caspases

KW - death receptor pathway

KW - proteosome

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JF - Journal of Surgical Research

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Screening and Detection of Apoptosis

Abstract

Keywords

ASJC Scopus subject areas

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