Safe pseudovirusbased assay for neutralization antibodies against influenza A(H7N9) virus

Chao Qiu; Yang Huang; Anli Zhang; Di Tian; Yanmin Wan; Xiaoling Zhang; Wanju Zhang; Zhiyong Zhang; Zhenghong Yuan; Yunwen Hu; Xiaoyan Zhang; Jianqing Xu

doi:10.3201/eid1910.130728

Safe pseudovirusbased assay for neutralization antibodies against influenza A(H7N9) virus

Chao Qiu, Yang Huang, Anli Zhang, Di Tian, Yanmin Wan, Xiaoling Zhang, Wanju Zhang, Zhiyong Zhang, Zhenghong Yuan, Yunwen Hu, Xiaoyan Zhang, Jianqing Xu

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus-based neutralization assay with preliminary assessment using subtype H7N9-infected samples and controls.

Original language	English (US)
Pages (from-to)	1685-1687
Number of pages	3
Journal	Emerging Infectious Diseases
Volume	19
Issue number	10
DOIs	https://doi.org/10.3201/eid1910.130728
State	Published - Oct 2013
Externally published	Yes

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

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10.3201/eid1910.130728

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title = "Safe pseudovirusbased assay for neutralization antibodies against influenza A(H7N9) virus",

abstract = "Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus-based neutralization assay with preliminary assessment using subtype H7N9-infected samples and controls.",

author = "Chao Qiu and Yang Huang and Anli Zhang and Di Tian and Yanmin Wan and Xiaoling Zhang and Wanju Zhang and Zhiyong Zhang and Zhenghong Yuan and Yunwen Hu and Xiaoyan Zhang and Jianqing Xu",

year = "2013",

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T1 - Safe pseudovirusbased assay for neutralization antibodies against influenza A(H7N9) virus

AU - Qiu, Chao

AU - Huang, Yang

AU - Zhang, Anli

AU - Tian, Di

AU - Wan, Yanmin

AU - Zhang, Xiaoling

AU - Zhang, Wanju

AU - Zhang, Zhiyong

AU - Yuan, Zhenghong

AU - Hu, Yunwen

AU - Zhang, Xiaoyan

AU - Xu, Jianqing

PY - 2013/10

Y1 - 2013/10

N2 - Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus-based neutralization assay with preliminary assessment using subtype H7N9-infected samples and controls.

AB - Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus-based neutralization assay with preliminary assessment using subtype H7N9-infected samples and controls.

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JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

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ER -

Safe pseudovirusbased assay for neutralization antibodies against influenza A(H7N9) virus

Abstract

ASJC Scopus subject areas

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