TY - JOUR
T1 - Role of integrin receptors in manganese-dependent BHK cell spreading on albumin-coated substrata
AU - Grinnell, Frederick
AU - Backman, Richard
N1 - Funding Information:
We are indebted to Dr. William Snell for his advice and suggestions regarding our studies. This research was supported by a grant from the NIH, CAl4609.
PY - 1991/7
Y1 - 1991/7
N2 - In manganese-containing medium, tissue cells can spread on albumin and other substrata typically nonadhesive for cells in calcium/magnesium-containing medium. To learn whether integrin receptors play a role in Mn-dependent adhesion, we tested the effects of RGD peptides and polyclonal anti-fibronectin receptor antibodies on BHK cell spreading on fibronectin and albumin-coated substrata. In Ca/Mg-containing medium on fibronectin substrata, the RGD-related peptides GRGDSP and GRGDS but not RGDS inhibited cell spreading. In Mn-containing medium, spreading on albumin was inhibited by GRGDSP and GRGDS and also by RGDS. GRGESP, on the other hand, did not inhibit cell spreading under any condition tested. Antibodies directed against fibronectin receptors also inhibited Mn-dependent cell spreading on albumin substrata, but higher levels of antibody were required than were necessary to inhibit Ca/Mg-dependent spreading on fibronectin. On the basis of these results, we suggest that integrin receptors, but probably not fibronectin receptors, mediate Mn-dependent BHK cell spreading on albumin.
AB - In manganese-containing medium, tissue cells can spread on albumin and other substrata typically nonadhesive for cells in calcium/magnesium-containing medium. To learn whether integrin receptors play a role in Mn-dependent adhesion, we tested the effects of RGD peptides and polyclonal anti-fibronectin receptor antibodies on BHK cell spreading on fibronectin and albumin-coated substrata. In Ca/Mg-containing medium on fibronectin substrata, the RGD-related peptides GRGDSP and GRGDS but not RGDS inhibited cell spreading. In Mn-containing medium, spreading on albumin was inhibited by GRGDSP and GRGDS and also by RGDS. GRGESP, on the other hand, did not inhibit cell spreading under any condition tested. Antibodies directed against fibronectin receptors also inhibited Mn-dependent cell spreading on albumin substrata, but higher levels of antibody were required than were necessary to inhibit Ca/Mg-dependent spreading on fibronectin. On the basis of these results, we suggest that integrin receptors, but probably not fibronectin receptors, mediate Mn-dependent BHK cell spreading on albumin.
UR - http://www.scopus.com/inward/record.url?scp=0025776028&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025776028&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(91)90520-5
DO - 10.1016/0014-4827(91)90520-5
M3 - Article
C2 - 1829042
AN - SCOPUS:0025776028
SN - 0014-4827
VL - 195
SP - 218
EP - 223
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -