Role of immersion refractometry for investigating laser‐induced effects in cells

Jerri Tribble, John Kozub, Al Aly, Robert Ossoff, Glenn Edwards

Research output: Contribution to journalArticlepeer-review


The broad background of scattered light observed in spectra of cell suspensions is reduced by factors of up to 20 by immersion refractometry allowing for improved spectroscopic determination of the absorption properties of cells in the 325–820 nm range. Refractive‐index matched spectra of E. coli C1a exhibit a set of resonant features near 422, 561, and 582 nm. Exposure wavelengths are chosen based on this spectrum and cell viability is investigated in E. coli suspensions exposed to 350, 400, 422, 440, and 700 nm radiation delivered in nanosecond pulses with total doses from 500 millijoules to 60 Joules. We observe a loss in cell viability for doses greater than 1 Joule at 422 nm and for all doses at other wavelengths; exposures of less than 1 Joule at 422 nm enhance growth. Excluding exposures at wavelengths within the resonant feature, longer wavelengths are less effective at reducing the viability of E. coli C1a. This indicates the occurrence of at least two absorption processes. © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)459-463
Number of pages5
JournalLasers in Surgery and Medicine
Issue number4
StatePublished - 1992
Externally publishedYes


  • Soret Band
  • bacteriostasis
  • phototherapy
  • porphyrin complexes
  • tunable‐dye laser

ASJC Scopus subject areas

  • Surgery
  • Dermatology


Dive into the research topics of 'Role of immersion refractometry for investigating laser‐induced effects in cells'. Together they form a unique fingerprint.

Cite this