TY - JOUR
T1 - Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes.
AU - Schlossman, D.
AU - Withers, D.
AU - Welsh, P.
AU - Alexander, A.
AU - Robertus, J.
AU - Frankel, A.
PY - 1989/11
Y1 - 1989/11
N2 - The gene for the A chain of ricin toxin was fused to a beta-galactosidase marker cistron via a DNA sequence encoding a short collagen linker, and the tripartite fusion protein was expressed in Escherichia coli. Site-specific mutagenesis was used to change glutamic acid residue 177 to aspartic acid or alanine. When the mutant proteins were expressed, purified, and tested quantitatively for enzymatic activity, the carboxylate function at position 177 was found not to be absolutely essential for ricin toxin A-chain catalysis.
AB - The gene for the A chain of ricin toxin was fused to a beta-galactosidase marker cistron via a DNA sequence encoding a short collagen linker, and the tripartite fusion protein was expressed in Escherichia coli. Site-specific mutagenesis was used to change glutamic acid residue 177 to aspartic acid or alanine. When the mutant proteins were expressed, purified, and tested quantitatively for enzymatic activity, the carboxylate function at position 177 was found not to be absolutely essential for ricin toxin A-chain catalysis.
UR - http://www.scopus.com/inward/record.url?scp=0024756611&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024756611&partnerID=8YFLogxK
U2 - 10.1128/MCB.9.11.5012
DO - 10.1128/MCB.9.11.5012
M3 - Article
C2 - 2689871
AN - SCOPUS:0024756611
SN - 0270-7306
VL - 9
SP - 5012
EP - 5021
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 11
ER -