Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A

Toshiki Itoh; Tadahiro Shiomi; Naoko Shiomi; Yoshinobu Harada; Mitsuo Wakasugi; Tsukasa Matsunaga; Osamu Nikaido; Errol C. Friedberg; Masaru Yamaizumi

doi:10.1016/0921-8777(95)00046-1

Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A

Toshiki Itoh, Tadahiro Shiomi, Naoko Shiomi, Yoshinobu Harada, Mitsuo Wakasugi, Tsukasa Matsunaga, Osamu Nikaido, Errol C. Friedberg, Masaru Yamaizumi

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

US31 is a UV-sensitive mutant cell line (rodent complementation group 8) derived from a mouse T cell line L5178Y. We analyzed removal kinetics for UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in US31 cells using monoclonal antibodies against these photoproducts. While nearly all (6-4) photoproducts were repaired within 6 h after UV-irradiation, more than 70% of cyclobutane pyrimidine dimers remained unrepaired even 24 h after UV-irradiation. These kinetics resembled those of Cockayne syndrome (CS) cells. Since US31 cells had a low efficiency of cell fusion and transfection, which hampered both complementation tests and gene cloning, we constructed fibroblastic complementation group 8 cell line 6L1030 by fusion of US31 cells with X-irradiated normal mouse fibroblastic LTA cells. Complementation tests by cell fusion and transfection using 6L1030 cells revealed that rodent complementation group 8 corresponded to CS complementation group A.

Original language	English (US)
Pages (from-to)	167-174
Number of pages	8
Journal	Mutation Research - DNA Repair
Volume	362
Issue number	2
DOIs	https://doi.org/10.1016/0921-8777(95)00046-1
State	Published - Feb 15 1996

Keywords

(6-4) photoproduct
Cockayne syndrome
Complementation test
Thymine dimer
Xeroderma pigmentosum

ASJC Scopus subject areas

Molecular Biology
Toxicology
Genetics

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10.1016/0921-8777(95)00046-1

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@article{893c8f20b84248018af38376e1963284,

title = "Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A",

abstract = "US31 is a UV-sensitive mutant cell line (rodent complementation group 8) derived from a mouse T cell line L5178Y. We analyzed removal kinetics for UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in US31 cells using monoclonal antibodies against these photoproducts. While nearly all (6-4) photoproducts were repaired within 6 h after UV-irradiation, more than 70% of cyclobutane pyrimidine dimers remained unrepaired even 24 h after UV-irradiation. These kinetics resembled those of Cockayne syndrome (CS) cells. Since US31 cells had a low efficiency of cell fusion and transfection, which hampered both complementation tests and gene cloning, we constructed fibroblastic complementation group 8 cell line 6L1030 by fusion of US31 cells with X-irradiated normal mouse fibroblastic LTA cells. Complementation tests by cell fusion and transfection using 6L1030 cells revealed that rodent complementation group 8 corresponded to CS complementation group A.",

keywords = "(6-4) photoproduct, Cockayne syndrome, Complementation test, Thymine dimer, Xeroderma pigmentosum",

author = "Toshiki Itoh and Tadahiro Shiomi and Naoko Shiomi and Yoshinobu Harada and Mitsuo Wakasugi and Tsukasa Matsunaga and Osamu Nikaido and Friedberg, {Errol C.} and Masaru Yamaizumi",

note = "Funding Information: We are grateful to Mrs. Yuka Itoh for preparation of the manuscript. This work was supported by grants from the Ministry of Education, Science and Culture of Japan and the Okukubo Memorial Fund for Medical Research in Kumamoto University School of Medicine.",

year = "1996",

month = feb,

day = "15",

doi = "10.1016/0921-8777(95)00046-1",

language = "English (US)",

volume = "362",

pages = "167--174",

journal = "Mutation Research - DNA Repair",

issn = "0921-8777",

publisher = "Elsevier BV",

number = "2",

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TY - JOUR

T1 - Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A

AU - Itoh, Toshiki

AU - Shiomi, Tadahiro

AU - Shiomi, Naoko

AU - Harada, Yoshinobu

AU - Wakasugi, Mitsuo

AU - Matsunaga, Tsukasa

AU - Nikaido, Osamu

AU - Friedberg, Errol C.

AU - Yamaizumi, Masaru

N1 - Funding Information: We are grateful to Mrs. Yuka Itoh for preparation of the manuscript. This work was supported by grants from the Ministry of Education, Science and Culture of Japan and the Okukubo Memorial Fund for Medical Research in Kumamoto University School of Medicine.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - US31 is a UV-sensitive mutant cell line (rodent complementation group 8) derived from a mouse T cell line L5178Y. We analyzed removal kinetics for UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in US31 cells using monoclonal antibodies against these photoproducts. While nearly all (6-4) photoproducts were repaired within 6 h after UV-irradiation, more than 70% of cyclobutane pyrimidine dimers remained unrepaired even 24 h after UV-irradiation. These kinetics resembled those of Cockayne syndrome (CS) cells. Since US31 cells had a low efficiency of cell fusion and transfection, which hampered both complementation tests and gene cloning, we constructed fibroblastic complementation group 8 cell line 6L1030 by fusion of US31 cells with X-irradiated normal mouse fibroblastic LTA cells. Complementation tests by cell fusion and transfection using 6L1030 cells revealed that rodent complementation group 8 corresponded to CS complementation group A.

AB - US31 is a UV-sensitive mutant cell line (rodent complementation group 8) derived from a mouse T cell line L5178Y. We analyzed removal kinetics for UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in US31 cells using monoclonal antibodies against these photoproducts. While nearly all (6-4) photoproducts were repaired within 6 h after UV-irradiation, more than 70% of cyclobutane pyrimidine dimers remained unrepaired even 24 h after UV-irradiation. These kinetics resembled those of Cockayne syndrome (CS) cells. Since US31 cells had a low efficiency of cell fusion and transfection, which hampered both complementation tests and gene cloning, we constructed fibroblastic complementation group 8 cell line 6L1030 by fusion of US31 cells with X-irradiated normal mouse fibroblastic LTA cells. Complementation tests by cell fusion and transfection using 6L1030 cells revealed that rodent complementation group 8 corresponded to CS complementation group A.

KW - (6-4) photoproduct

KW - Cockayne syndrome

KW - Complementation test

KW - Thymine dimer

KW - Xeroderma pigmentosum

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Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A

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