Abstract
Detailed characterization of cellular RNA facilitates the design of nucleic acid therapeutics and interpretation of experimental data. Since the first reports of next generation sequencing (NGS) technology-based RNA sequencing (RNA-seq) (Nagalakshmi et al., 2008; Wilhelm, et al., 2008), rapid advances in experimental protocol development and data acquisition and analysis have brought comprehensive sequencing of cellular RNA within reach of most laboratories (Martin and Wang, 2011; Ozsolak and Milos, 2011). While RNA-seq is becoming widely used for laboratory research and clinical studies, the field is still new and strategies for successfully applying RNA-seq to a given experimental problem are often obscure. The goal of this perspective is to introduce some of the choices confronted during RNA sequencing and analysis.
Original language | English (US) |
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Pages (from-to) | 271-274 |
Number of pages | 4 |
Journal | Nucleic Acid Therapeutics |
Volume | 22 |
Issue number | 4 |
DOIs | |
State | Published - Aug 1 2012 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Genetics
- Drug Discovery